Mesenchylmal Stem Cell Culture on Poly(N-isopropylacrylamide) Hydrogel with Repeated Thermo-Stimulation

Aya Mizutani Akimoto; Erika Hasuike Niitsu; Kenichi Nagase; Teruo Okano; Hideko Kanazawa; Ryo Yoshida

doi:10.3390/ijms19041253

Mesenchylmal Stem Cell Culture on Poly(N-isopropylacrylamide) Hydrogel with Repeated Thermo-Stimulation

Int J Mol Sci. 2018 Apr 21;19(4):1253. doi: 10.3390/ijms19041253.

Authors

Aya Mizutani Akimoto¹, Erika Hasuike Niitsu², Kenichi Nagase³, Teruo Okano⁴, Hideko Kanazawa⁵, Ryo Yoshida⁶

Affiliations

¹ Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, Japan. akimoto@cross.t.u-tokyo.ac.jp.
² Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato, Tokyo 105-8512, Japan. hasu.eri523@gmail.com.
³ Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku, Tokyo 162-8666, Japan. nagase-kn@pha.keio.ac.jp.
⁴ Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku, Tokyo 162-8666, Japan. tokano@twmu.ac.jp.
⁵ Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato, Tokyo 105-8512, Japan. kanazawa-hd@pha.keio.ac.jp.
⁶ Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, Japan. ryo@cross.t.u-tokyo.ac.jp.

Abstract

We prepared thermoresponsive hydrogels by mixing poly(N-isopropylacrylamide) (PNIPAAm) derivatives as the main chain components, octa-arm polyethylene glycol (PEG) as a crosslinker, and the Arg-Gly-Asp-Ser (RGDS) peptides as cell adhesion units. Human bone marrow-derived mesenchymal stem cells (hbmMSCs) were cultured on the hydrogels. The PNIPAAm gel prepared by the post-crosslinking gelation method was revealed to be cytocompatible and showed temperature-dependent changes in mechanical properties. Repeated changes in the swelling ratio of the PNIPAAm gel affected the shape of the hbmMSCs. With respect to both cytocompatibility and reversibility of changes in mechanical properties, the PNIPAAm gel system could be potentially useful for the analysis of cell mechanobiology.

Keywords: cell culture; hydrogel; mechanobiology; mesenchymal stem cell; poly(N-isopropylacrylamide).

MeSH terms

Acrylic Resins / chemistry*
Biophysics / methods
Cells, Cultured
Humans
Hydrogels / chemistry*
Mesenchymal Stem Cells / physiology*
Polyethylene Glycols / chemistry
Temperature
Tissue Engineering / methods

Substances

Acrylic Resins
Hydrogels
poly-N-isopropylacrylamide
Polyethylene Glycols