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Page 1
Prognostic biomarkers of IFNb therapy in multiple sclerosis patients.
Baranzini SE, Madireddy LR, Cromer A, D'Antonio M, Lehr L, Beelke M, Farmer P, Battaglini M, Caillier SJ, Stromillo ML, De Stefano N, Monnet E, Cree BA. Baranzini SE, et al. Mult Scler. 2015 Jun;21(7):894-904. doi: 10.1177/1352458514555786. Epub 2014 Nov 12. Mult Scler. 2015. PMID: 25392319 Clinical Trial.
Detection and kinetics of persistent neutralizing anti-interferon-beta antibodies in patients with multiple sclerosis. Results from the ABIRISK prospective cohort study.
Jensen PEH, Warnke C, Ingenhoven K, Piccoli L, Gasis M, Hermanrud C, Fernandez-Rodriguez BM, Ryner M, Kramer D, Link J, Ramanujam R, Auer M, Buck D, Grummel V, Bertotti E, Fissolo N, Oliver-Martos B, Nytrova P, Khalil M, Guger M, Rathmaier S, Sievers-Stober C, Lindberg RLP, Hässler S, Bachelet D, Aktas O, Donnellan N, Lawton A, Hemmer B, Havrdova EK, Kieseier B, Hartung HP, Comabella M, Montalban X, Derfuss T, Sellebjerg F, Dönnes P, Pallardy M, Spindeldreher S, Broët P, Deisenhammer F, Fogdell-Hahn A, Sorensen PS; ABIRISK Consortium. Jensen PEH, et al. J Neuroimmunol. 2019 Jan 15;326:19-27. doi: 10.1016/j.jneuroim.2018.11.002. Epub 2018 Nov 7. J Neuroimmunol. 2019. PMID: 30447419
Two validated assays, a bridging ELISA and a luciferase-based bioassay, were compared for detection of anti-drug antibodies (ADA) against interferon-beta (IFN-beta) in patients with multiple sclerosis. ...For clinical samples, selection of method of ELISA should be …
Two validated assays, a bridging ELISA and a luciferase-based bioassay, were compared for detection of anti-drug antibodies (ADA) aga …
Validation of a mass spectrometry-based method for milk traces detection in baked food.
Lamberti C, Acquadro E, Corpillo D, Giribaldi M, Decastelli L, Garino C, Arlorio M, Ricciardi C, Cavallarin L, Giuffrida MG. Lamberti C, et al. Food Chem. 2016 May 15;199:119-27. doi: 10.1016/j.foodchem.2015.11.130. Epub 2015 Nov 30. Food Chem. 2016. PMID: 26775952
Method applicability was evaluated by analysing commercial cookies labelled either as "milk-free" or "may contain milk". Although the ELISA methodology is considered the gold standard for detecting allergens in foods, this robust LC-MS/MS approach should be a useful confir …
Method applicability was evaluated by analysing commercial cookies labelled either as "milk-free" or "may contain milk". Although the ELI …
Efficacy of a Test-Retest Strategy in Residents and Health Care Personnel of a Nursing Home Facing a COVID-19 Outbreak.
Blain H, Rolland Y, Tuaillon E, Giacosa N, Albrand M, Jaussent A, Benetos A, Miot S, Bousquet J. Blain H, et al. J Am Med Dir Assoc. 2020 Jul;21(7):933-936. doi: 10.1016/j.jamda.2020.06.013. Epub 2020 Jun 11. J Am Med Dir Assoc. 2020. PMID: 32674822 Free PMC article.
Six weeks after initial testing, all residents underwent testing for enzyme-linked immunosorbent assay-based IgG antibodies directed against the SARS-CoV-2. ...
Six weeks after initial testing, all residents underwent testing for enzyme-linked immunosorbent assay-based IgG …
Probing treatment response of glutaminolytic prostate cancer cells to natural drugs with hyperpolarized [5-(13) C]glutamine.
Canapè C, Catanzaro G, Terreno E, Karlsson M, Lerche MH, Jensen PR. Canapè C, et al. Magn Reson Med. 2015 Jun;73(6):2296-305. doi: 10.1002/mrm.25360. Epub 2014 Jul 9. Magn Reson Med. 2015. PMID: 25045880 Free article.
METHODS: Hyperpolarized [5-(13) C]glutamine is used to measure glutamine metabolism in two prostate cancer cell lines (PC3 and DU145) before and after treatment with the two natural anticancer drugs resveratrol and sulforaphane. An invasive biochemical assay simulating the …
METHODS: Hyperpolarized [5-(13) C]glutamine is used to measure glutamine metabolism in two prostate cancer cell lines (PC3 and DU145) before …
Cloning, expression, and antigenic characterization of recombinant protein of Mycoplasma gallisepticum expressed in Escherichia coli.
Rocha TS, Tramuta C, Catania S, Matucci A, Giuffrida MG, Baro C, Profiti M, Bertolotti L, Rosati S. Rocha TS, et al. Poult Sci. 2015 Apr;94(4):621-7. doi: 10.3382/ps/pev012. Epub 2015 Feb 9. Poult Sci. 2015. PMID: 25667423 Free article.
., E2) protein by 2-dimensional electrophoresis (2-DE), characterized it in immunoblotting assays, and analyzed its recombinant (r-E2) in a rec-ELISA test. For full-length protein expression in Escherichia coli (EC) a point mutation was introduced. ...Also, anti-E2 serum r …
., E2) protein by 2-dimensional electrophoresis (2-DE), characterized it in immunoblotting assays, and analyzed its recombinant (r-E2) in a …
Antifibroblast antibodies from systemic sclerosis patients are internalized by fibroblasts via a caveolin-linked pathway.
Ronda N, Gatti R, Giacosa R, Raschi E, Testoni C, Meroni PL, Buzio C, Orlandini G. Ronda N, et al. Arthritis Rheum. 2002 Jun;46(6):1595-601. doi: 10.1002/art.10362. Arthritis Rheum. 2002. PMID: 12115191 Free article.
METHODS: We coupled to fluorescein 1) IgG purified from AFA-positive and AFA-negative SSc sera (as assessed by cellular enzyme-linked immunosorbent assay) and 2) single healthy donor and pooled normal IgG. ...
METHODS: We coupled to fluorescein 1) IgG purified from AFA-positive and AFA-negative SSc sera (as assessed by cellular enzyme-lin …
Normal human IgG prevents endothelial cell activation induced by TNFalpha and oxidized low-density lipoprotein atherogenic stimuli.
Ronda N, Bernini F, Giacosa R, Gatti R, Baldini N, Buzio C, Orlandini G. Ronda N, et al. Clin Exp Immunol. 2003 Aug;133(2):219-26. doi: 10.1046/j.1365-2249.2003.02215.x. Clin Exp Immunol. 2003. PMID: 12869027 Free PMC article.
Confocal microscopy was used to visualize vascular cell adhesion molecule-1 (CD106) expression on endothelial cells, cytoplasmic free calcium ([Ca++]i) modifications and fluorescein-coupled oxLDL internalization. Cytokine secretion was measured by ELISA on cell supernatant …
Confocal microscopy was used to visualize vascular cell adhesion molecule-1 (CD106) expression on endothelial cells, cytoplasmic free calciu …
Reduction of whey protein concentrate antigenicity by using a combined enzymatic digestion and ultrafiltration approach.
Quintieri L, Monaci L, Baruzzi F, Giuffrida MG, de Candia S, Caputo L. Quintieri L, et al. J Food Sci Technol. 2017 Jun;54(7):1910-1916. doi: 10.1007/s13197-017-2625-5. Epub 2017 Apr 28. J Food Sci Technol. 2017. PMID: 28720947 Free PMC article.
The electrophoretic profiles of both pepsin and papain WPC hydrolysates proved the disappearance of the BLG band, even though a slight antigenicity was still found by ELISA. Pepsin hydrolysates, filtered through a 10-kDa cut-off membrane, did not produce immunological resp …
The electrophoretic profiles of both pepsin and papain WPC hydrolysates proved the disappearance of the BLG band, even though a slight antig …
Identification of the human beta-casein C-terminal fragments that specifically bind to purified antibodies to bovine beta-lactoglobulin.
Conti A, Giuffrida MG, Napolitano L, Quaranta S, Bertino E, Coscia A, Costa S, Fabris C. Conti A, et al. J Nutr Biochem. 2000 Jun;11(6):332-7. doi: 10.1016/s0955-2863(00)00086-3. J Nutr Biochem. 2000. PMID: 11002129
Moreover, a strong cross-reactivity between antibodies to bovine beta-LG and human milk proteins and peptides was reported, throwing doubt on the reliability of radioimmunoassay and enzyme-linked immunosorbent assay detection and quantification assays …
Moreover, a strong cross-reactivity between antibodies to bovine beta-LG and human milk proteins and peptides was reported, throwing doubt o …