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Efficient soluble expression of active recombinant human cyclin A2 mediated by E. coli molecular chaperones.
Grigoroudis AI, McInnes C, Premnath PN, Kontopidis G. Grigoroudis AI, et al. Protein Expr Purif. 2015 Sep;113:8-16. doi: 10.1016/j.pep.2015.01.013. Epub 2015 May 6. Protein Expr Purif. 2015. PMID: 25956535 Free PMC article.
A high yield of human cyclin A2 was obtained in natively folded and soluble form, through co-expression with groups of molecular chaperones from E. coli in various combinations. A one-step affinity chromatography method was utilized to purify the fusion protein products to …
A high yield of human cyclin A2 was obtained in natively folded and soluble form, through co-expression with groups of molecular chaperones …
Phosphorylation activity of the response regulator of the two-component signal transduction system AtoS-AtoC in E. coli.
Lioliou EE, Mimitou EP, Grigoroudis AI, Panagiotidis CH, Panagiotidis CA, Kyriakidis DA. Lioliou EE, et al. Biochim Biophys Acta. 2005 Oct 10;1725(3):257-68. doi: 10.1016/j.bbagen.2005.06.019. Biochim Biophys Acta. 2005. PMID: 16153782
This operon encodes enzymes involved in short-chain fatty acid catabolism in E. coli. Evidence is presented to show that AtoS is a sensor kinase that together with AtoC constitutes a two-component signal transduction system. ...
This operon encodes enzymes involved in short-chain fatty acid catabolism in E. coli. Evidence is presented to show that AtoS is a se …
S-MEDUTA: Combining Balanced Scorecard with Simulation and MCDA Techniques for the Evaluation of the Strategic Performance of an Emergency Department.
Manolitzas P, Grigoroudis E, Christodoulou J, Matsatsinis N. Manolitzas P, et al. Adv Exp Med Biol. 2020;1194:1-22. doi: 10.1007/978-3-030-32622-7_1. Adv Exp Med Biol. 2020. PMID: 32468519
Through the simulation model, the stakeholders have the ability to evaluate the effect of their decisions on a number of KPIs that are important for the successful implementation of strategy on the ED. This method is able to provide a set of completed results (e.g., scores …
Through the simulation model, the stakeholders have the ability to evaluate the effect of their decisions on a number of KPIs that are impor …
Escherichia coli genome-wide promoter analysis: identification of additional AtoC binding target elements.
Pilalis E, Chatziioannou AA, Grigoroudis AI, Panagiotidis CA, Kolisis FN, Kyriakidis DA. Pilalis E, et al. BMC Genomics. 2011 May 13;12(1):238. doi: 10.1186/1471-2164-12-238. BMC Genomics. 2011. PMID: 21569465 Free PMC article.
BACKGROUND: Studies on bacterial signal transduction systems have revealed complex networks of functional interactions, where the response regulators play a pivotal role. The AtoSC system of E. coli activates the expression of atoDAEB operon genes, and the subsequent catab …
BACKGROUND: Studies on bacterial signal transduction systems have revealed complex networks of functional interactions, where the response r …
Quantification of the effects of ionic strength, viscosity, and hydrophobicity on protein-ligand binding affinity.
Papaneophytou CP, Grigoroudis AI, McInnes C, Kontopidis G. Papaneophytou CP, et al. ACS Med Chem Lett. 2014 Jul 9;5(8):931-6. doi: 10.1021/ml500204e. eCollection 2014 Aug 14. ACS Med Chem Lett. 2014. PMID: 25147617 Free PMC article.
Crucial data for ligand-protein binding elucidation is mainly derived from techniques (e.g., macromolecular crystallography) that require the addition of high concentration of salts and/or other additives. ...
Crucial data for ligand-protein binding elucidation is mainly derived from techniques (e.g., macromolecular crystallography) that req …