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Reduced protease activity in transformed rice cell suspension cultures expressing a proteinase inhibitor.
Kim TG, Kim HM, Lee HJ, Shin YJ, Kwon TH, Lee NJ, Jang YS, Yang MS. Kim TG, et al. Protein Expr Purif. 2007 Jun;53(2):270-4. doi: 10.1016/j.pep.2007.01.005. Epub 2007 Jan 18. Protein Expr Purif. 2007. PMID: 17314052
In an effort to reduce protease activity in a rice cell suspension culture, we first synthesized sPI-II using overlap PCR and then introduced the gene into a rice calli (Oryza sativa L. cv. Dongin) by particle bombardment-mediated transformation. The sPI-II gene was under …
In an effort to reduce protease activity in a rice cell suspension culture, we first synthesized sPI-II using overlap PCR and then introduce …