Ultraviolet C irradiation induces different expression of cyclooxygenase 2 in NIH 3T3 cells and A431 cells: the roles of COX-2 are different in various cell lines

Int J Mol Sci. 2012;13(4):4351-4366. doi: 10.3390/ijms13044351. Epub 2012 Apr 5.

Abstract

Ultraviolet C (UVC) is a DNA damage inducer, and 20 J/m(2) of UVC irradiation caused cell growth inhibition and induced cell death after exposure for 24-36 h. The growth of NIH 3T3 cells was significantly suppressed at 24 h after UVC irradiation whereas the proliferation of A431 cells was inhibited until 36 h after UVC irradiation. UVC irradiation increased COX-2 expression and such up-regulation reached a maximum during 3-6 h in NIH 3T3 cells. In contrast, UVC-induced COX-2 reached a maximum after 24-36 h in A431 cells. Measuring prostaglandin E2 (PGE2) level showed a biphasic profile that PGE2 release was rapidly elevated in 1-12 h after UVC irradiation and increased again at 24 h in both cell lines. Treatment with the selective COX-2 inhibitor, SC-791, during maximum expression of COX-2 induction, attenuated the UVC induced-growth inhibition in NIH 3T3 cells. In contrast, SC-791 treatment after UVC irradiation enhanced death of A431 cells. These data showed that the patterns of UVC-induced PGE2 secretion from NIH 3T3 cells and A431 cells were similar despite the differential profile in UVC-induced COX-2 up-regulation. Besides, COX-2 might play different roles in cellular response to UVC irradiation in various cell lines.

Keywords: A431 cells; NIH 3T3 cells; cyclooxygenases-2 (COX-2); prostaglandin E2 (PGE2); ultraviolet C (UVC).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Animals
  • Apoptosis / drug effects
  • Apoptosis / radiation effects
  • Cell Line
  • Cell Proliferation / drug effects
  • Cell Proliferation / radiation effects
  • Cyclooxygenase 2 / biosynthesis*
  • Cyclooxygenase 2 Inhibitors / pharmacology
  • Dinoprostone / biosynthesis*
  • Dinoprostone / metabolism
  • Humans
  • Isoxazoles / pharmacology*
  • Mice
  • Sulfonamides / pharmacology*
  • Ultraviolet Rays / adverse effects*
  • Up-Regulation / radiation effects

Substances

  • Cyclooxygenase 2 Inhibitors
  • Isoxazoles
  • SC 791
  • Sulfonamides
  • Cyclooxygenase 2
  • Dinoprostone