Identification and Evaluation of Cancer Stem Cells in Oral Squamous Cell Carcinoma and Oral Epithelial Dysplasia Using NANOG: An Immunohistochemical Study

Arya I; Varun B Raghavan Pillai; Anna P Joseph; Pratibha Ramani; Jayanthi P; Karthikeyan Ramalingam

doi:10.7759/cureus.55111

Identification and Evaluation of Cancer Stem Cells in Oral Squamous Cell Carcinoma and Oral Epithelial Dysplasia Using NANOG: An Immunohistochemical Study

Cureus. 2024 Feb 27;16(2):e55111. doi: 10.7759/cureus.55111. eCollection 2024 Feb.

Authors

Arya I¹, Varun B Raghavan Pillai^{2

1}, Anna P Joseph¹, Pratibha Ramani², Jayanthi P³, Karthikeyan Ramalingam²

Affiliations

¹ Oral and Maxillofacial Pathology, PMS College of Dental Science and Research, Trivandrum, IND.
² Oral Pathology and Microbiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
³ Oral and Maxillofacial Pathology, Azeezia College of Dental Sciences and Research, Kollam, IND.

Abstract

Background: Squamous cell carcinoma of the oral cavity may show precursor lesions, termed as potentially malignant disorders, of which leukoplakia is the most frequent one. Oral leukoplakia is a clinical diagnosis for which the histological diagnosis may be either hyperplasia or oral epithelial dysplasia (OED) and sometimes even oral squamous cell carcinoma (OSCC). Cancer stem cells (CSCs), identified in various tumors, are a specific group of cells that exhibit the properties of self-renewal and differentiation. Among the various biomarkers that identify CSCs, the transcription factor NANOG is considered to be a significant one.

Aim: In this study, we intend to identify and compare the immunohistochemical expression of NANOG in OSCC, OED, and normal oral mucosa.

Methodology: Tissue blocks of OSCC (n=28), OED (n=28), and normal oral mucosa (n=28) were used in this study. Specimens were immunohistochemically analyzed for NANOG expression. The results were statistically analyzed using one-way ANOVA, Games-Howell post hoc, and Student t-test. Statistical Product and Service Solutions (SPSS, version 21; IBM SPSS Statistics for Windows, Armonk, NY) software was used for performing the statistical analysis, and the level of significance was set as 0.05.

Observations: NANOG expression was higher in OSCC when compared to oral dysplasias and normal oral mucosa, in decreasing order. A significantly higher histo-score and labeling index score were observed in OSCC and oral dysplasias compared to normal oral mucosa (p=<0.001).

Conclusion: The expression levels of NANOG were positively correlated with disease progression in OSCC, implicating that NANOG can be used as a surrogate marker of oral oncogenesis and prognosis. Therefore, decoding the molecular mechanisms of NANOG regulation in the progression of cancer helps in developing new therapeutic strategies for oral cancer.

Keywords: cancer stem cells; expression; immunohistochemistry; marker; oral epithelial dysplasias; oral leukoplakia; oscc; potentially malignant; prognosis; progression.