Accelerated detection of viral particles by combining AC electric field effects and micro-Raman spectroscopy

Sensors (Basel). 2015 Jan 8;15(1):1047-59. doi: 10.3390/s150101047.

Abstract

A detection method that combines electric field-assisted virus capture on antibody-decorated surfaces with the "fingerprinting" capabilities of micro-Raman spectroscopy is demonstrated for the case of M13 virus in water. The proof-of-principle surface mapping of model bioparticles (protein coated polystyrene spheres) captured by an AC electric field between planar microelectrodes is presented with a methodology for analyzing the resulting spectra by comparing relative peak intensities. The same principle is applied to dielectrophoretically captured M13 phage particles whose presence is indirectly confirmed with micro-Raman spectroscopy using NeutrAvidin-Cy3 as a labeling molecule. It is concluded that the combination of electrokinetically driven virus sampling and micro-Raman based signal transduction provides a promising approach for time-efficient and in situ detection of viruses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophage M13 / isolation & purification*
  • Electricity*
  • Microelectrodes
  • Polystyrenes / chemistry
  • Silicon / chemistry
  • Spectrum Analysis, Raman / methods*
  • Virion / isolation & purification*

Substances

  • Polystyrenes
  • Silicon