Copaiba oil suppresses inflammatory cytokines in splenocytes of C57Bl/6 mice induced with experimental autoimmune encephalomyelitis (EAE)

Débora S Dias; Lívia B A Fontes; Antônio E M Crotti; Beatriz J V Aarestrup; Fernando M Aarestrup; Ademar A da Silva Filho; José O A Corrêa

doi:10.3390/molecules190812814

Copaiba oil suppresses inflammatory cytokines in splenocytes of C57Bl/6 mice induced with experimental autoimmune encephalomyelitis (EAE)

Molecules. 2014 Aug 21;19(8):12814-26. doi: 10.3390/molecules190812814.

Authors

Débora S Dias¹, Lívia B A Fontes¹, Antônio E M Crotti², Beatriz J V Aarestrup³, Fernando M Aarestrup³, Ademar A da Silva Filho⁴, José O A Corrêa⁵

Affiliations

¹ Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Juiz de Fora, R. José Lourenço Kelmer s/n, Campus Universitário, 36036-900 Juiz de Fora, MG, Brazil.
² Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, 14040-901 Ribeirão Preto, SP, Brazil.
³ Laboratory of Experimental Imunology and Patology, CBR, Federal University of Juiz de Fora, Juiz de Fora, R. José Lourenço Kelmer s/n, Campus Universitário, 36036-900 Juiz de Fora, MG, Brazil.
⁴ Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Juiz de Fora, R. José Lourenço Kelmer s/n, Campus Universitário, 36036-900 Juiz de Fora, MG, Brazil. ademar.alves@ufjf.edu.br.
⁵ Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Juiz de Fora, R. José Lourenço Kelmer s/n, Campus Universitário, 36036-900 Juiz de Fora, MG, Brazil. joacorrea@gmail.com.

Abstract

Experimental autoimmune encephalomyelitis (EAE) is a murine autoimmune disease used to study multiple sclerosis. We have investigated the immunomodulatory effects of copaiba oil (100, 50 and 25 µg/mL) on NO, H2O2, TNF-α, IFN-γ and IL-17 production in cultured cells from EAE-mice. Copaiba oil (100 µg/mL) inhibited H2O2, NO, IFN-γ TNF-α and IL-17 production spontaneously or after ConA and MOG35-55 stimulation. It is suggested that copaiba oil acts on the mechanism of development of EAE by IFN-γ, IL-17 and TNF-α inhibition, modulating the immune response on both Th1 and Th17 cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Inflammatory Agents / pharmacology*
Cells, Cultured
Cytokines / metabolism*
Drug Evaluation, Preclinical
Encephalomyelitis, Autoimmune, Experimental / drug therapy*
Encephalomyelitis, Autoimmune, Experimental / immunology
Fabaceae / chemistry
Female
Hydrogen Peroxide / metabolism
Mice, Inbred C57BL
Nitric Oxide / metabolism
Plant Oils / pharmacology*
Spleen / pathology*

Substances

Anti-Inflammatory Agents
Cytokines
Plant Oils
Nitric Oxide
Hydrogen Peroxide