Immunofluorescence vs immunochemiluminescent methods: AIA 2000 vs Immulite 2000

Clin Biochem. 2015 Nov;48(16-17):1184-7. doi: 10.1016/j.clinbiochem.2015.05.017. Epub 2015 Jun 3.

Abstract

The aim of this study was to evaluate the measurement of C-peptide, insulin, sex hormone binding globulin (SHBG), and dehydroepiandrosterone sulfate (DHEAS), tests of specific clinical value that are not frequently measured in routine clinical laboratories, carried out using an automated system, AIA 2000 (Tosoh). The obtained data demonstrated that the evaluated system, characterized by fluorescence system detector provides satisfactory analytical performance comparable to those of different instrument that use the well known and widely diffused chemiluminescent principle (Immulite 2000). These characteristics allow an excellent comparability between methods for the measurement of same specific hormones not frequently used in clinical practice.

Keywords: C-peptide; DHEA-S; Insulin; SHBG.

Publication types

  • Comparative Study

MeSH terms

  • C-Peptide / metabolism
  • Clinical Laboratory Techniques / methods*
  • Dehydroepiandrosterone Sulfate / metabolism
  • Fluorescent Antibody Technique / methods*
  • Humans
  • Insulin / metabolism
  • Sex Hormone-Binding Globulin / metabolism

Substances

  • C-Peptide
  • Insulin
  • Sex Hormone-Binding Globulin
  • Dehydroepiandrosterone Sulfate