In the present study, we have used seed extract of P. nigrum as an effective reducing agent for the synthesis of silver nanoparticles (Ag NPs) and silver based chitosan nanocomposite (Ag/CS NC). The silver nanoparticles were characterized by UV-vis spectroscopy, X-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM) and Fourier transform infrared spectroscopy (FTIR). The appearance of a surface plasmon resonance (SPR) peak located at 430 nm reveals the formation of silver nanoparticles. The TEM analysis indicates that the attained silver nanoparticles were mostly in spherical shapes with sizes ranging between 15 and 38 nm. The high resolution liquid chromatography with mass spectroscopy (HR-LCMS) analysis was performed to screen the phytochemical constituents of P. nigrum seed extract. The silver nanoparticles synthesized by P. nigrum seed extract exhibited effective antibacterial activity against Bacillus subtilis (B. subtilis) and Escherichia coli (E. coli). In addition, the silver nanoparticles showed potent cytotoxicity against hazardous human cancer cell lines MDA-MB-231, PANC-1, SKOV-3, PC-3 and Hela. The observation of bright spots in the TEM dark field images represents the presence of Ag in CS suspension. Besides, the Ag/CS NC coated cotton fabric substantially showed remarkable antibacterial activity against B. subtilis and E. coli.
Keywords: Green synthesis; Piper nigrum; Silver nanoparticles.
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