Assessment of Intestinal Immunity and Permeability of Broilers on Partial Replacement Diets of Two-Stage Fermented Soybean Meal by Bacillus velezensis and Lactobacillus brevis ATCC 367

Chia Fen Tsai; Li Jen Lin; Chao Humg Wang; Ching Sung Tsai; Shang Chang Chang; Tzu Tai Lee

doi:10.3390/ani11082336

Assessment of Intestinal Immunity and Permeability of Broilers on Partial Replacement Diets of Two-Stage Fermented Soybean Meal by Bacillus velezensis and Lactobacillus brevis ATCC 367

Animals (Basel). 2021 Aug 8;11(8):2336. doi: 10.3390/ani11082336.

Authors

Chia Fen Tsai¹, Li Jen Lin², Chao Humg Wang³, Ching Sung Tsai³, Shang Chang Chang⁴, Tzu Tai Lee^{1

5}

Affiliations

¹ Department of Animal Science, National Chung Hsing University, Taichung 402, Taiwan.
² School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung 404, Taiwan.
³ Central Union Oil Corporation, Taichung 436, Taiwan.
⁴ Kaohsiung Animal Propagation Station, Livestock Research Institute, Council of Agriculture, Kaohsiung 912, Taiwan.
⁵ The iEGG and Animal Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan.

Abstract

The effect of soybean peptides from fermented soybean meal on the intestinal immunity and tight junction of broilers was assessed. Roughly, two-stage fermented soybean meal prepared with Bv and Lb (FSBM_B+L), which has nearly three times higher soluble peptides than soybean meal (SBM), and reduced galacto-oligosaccharide (GOS) content and allergen protein. The one-stage fermented by Bv (FSBM_B) has the highest soluble peptides, while commercial lactic acid bacteria (FSBM_L) has the highest Lactic acid bacteria count; these were used to compare the differences in the process. Ross308 broilers (n = 320) were divided into four groups: SBM diet and a diet replaced with 6% FSBM_B+L, FSBM_B, or FSBM_L. The growth performance was recorded during the experiment, and six birds (35-day-old) per group were euthanized. Analysis of their jejunum and ileum showed that the fermented soybean meal significantly improved the villus height in the jejunum (p < 0.05) and reduced the crypt hyperplasia. The FSBM_B group had the highest reducing crypt depth; however, the FSBM_B+L group had the highest villus height/crypt depth in the ileum (p < 0.05). In the jejunum, the relative mRNA of CLDN-1 and Occludin increased 2-fold in the treatments, and ZO-1 mRNA increased 1.5 times in FSBM_L and FSBM_B+L (p < 0.05). Furthermore, the level of NF-κB and IL-6 mRNAs in FSBM_L increased, respectively, by 4 and 2.5 times. While FSBM_B, along with FSBM_B+L, had a 1.5-fold increase in the mRNA of IL-10, that of NF-κB increased 2-fold. FSBM_B+L and FSBM_B singly led to a 2- and 3-fold increase in IL-6 mRNA, respectively (p < 0.05). FSBM_B and FSBM_B+L can also upregulate MUC2 in the jejunum (p < 0.05). In short, using the soybean peptides from two-stage fermented soybean meal can ameliorate the negative factors of SBM and effectively regulate immune expression and intestinal repair, which will help broilers maintain intestinal integrity.

Keywords: broiler; immunity; intestinal morphology; soy peptide; soybean meal; tight junction; two-stage fermentation.