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Analysis of Cryopreservation Protocols and Their Harmful Effects on the Endothelial Integrity of Human Corneas.
Rodríguez-Fernández S, Álvarez-Portela M, Rendal-Vázquez E, Piñeiro-Ramil M, Sanjurjo-Rodríguez C, Castro-Viñuelas R, Sánchez-Ibáñez J, Fuentes-Boquete I, Díaz-Prado S. Rodríguez-Fernández S, et al. Int J Mol Sci. 2021 Nov 22;22(22):12564. doi: 10.3390/ijms222212564. Int J Mol Sci. 2021. PMID: 34830446 Free PMC article.
Cryoprotectant solutions of the TK protocol presented an increasing osmolality and a physiological pH value. Cooling temperature rate of TK protocol was of 1 C/min to -40 C and 3 C/min to -120 C, and almost all of dimethyl sulfoxide left the tissue aft …
Cryoprotectant solutions of the TK protocol presented an increasing osmolality and a physiological pH value. Cooling temperature rate of TK …
Generation and characterization of human induced pluripotent stem cells (iPSCs) from hand osteoarthritis patient-derived fibroblasts.
Castro-Viñuelas R, Sanjurjo-Rodríguez C, Piñeiro-Ramil M, Hermida-Gómez T, Rodríguez-Fernández S, Oreiro N, de Toro J, Fuentes I, Blanco FJ, Díaz-Prado S. Castro-Viñuelas R, et al. Sci Rep. 2020 Mar 6;10(1):4272. doi: 10.1038/s41598-020-61071-6. Sci Rep. 2020. PMID: 32144293 Free PMC article.
We performed a non-integrative reprogramming of dermal fibroblasts obtained from two patients with radiographic rhizarthrosis and non-erosive hOA by introducing the transcriptional factors Oct4, Sox2, Klf4 and c-Myc using Sendai virus. After reprogramming, embryonic stem c …
We performed a non-integrative reprogramming of dermal fibroblasts obtained from two patients with radiographic rhizarthrosis and non-erosiv …
Generation of a human control iPS cell line (ESi080-A) from a donor with no rheumatic diseases.
Castro-Viñuelas R, Sanjurjo-Rodríguez C, Piñeiro-Ramil M, Rodríguez-Fernández S, Fuentes-Boquete IM, Blanco FJ, Díaz-Prado SM. Castro-Viñuelas R, et al. Stem Cell Res. 2020 Mar;43:101683. doi: 10.1016/j.scr.2019.101683. Epub 2019 Dec 9. Stem Cell Res. 2020. PMID: 31962233 Free article.
Here, we report the establishment of the human iPS cell line N1-FiPS4F#7 generated from skin cells of a patient with no rheumatic diseases, thus obtaining an appropriate control iPS cell line for researchers working in the field of rheumatic diseases. The reprogramming factors Oc …
Here, we report the establishment of the human iPS cell line N1-FiPS4F#7 generated from skin cells of a patient with no rheumatic diseases, …
Gene Expression Signatures of Synovial Fluid Multipotent Stromal Cells in Advanced Knee Osteoarthritis and Following Knee Joint Distraction.
Sanjurjo-Rodriguez C, Altaie A, Mastbergen S, Baboolal T, Welting T, Lafeber F, Pandit H, McGonagle D, Jones E. Sanjurjo-Rodriguez C, et al. Front Bioeng Biotechnol. 2020 Oct 14;8:579751. doi: 10.3389/fbioe.2020.579751. eCollection 2020. Front Bioeng Biotechnol. 2020. PMID: 33178674 Free PMC article.
In KJD cohort (n = 9 patients), joint off-loading resulted in sustained, significant increase in SF MSC colonies' sizes and densities and a notable transcript upregulation of key cartilage core protein aggrecan (ACAN) (weeks 3 and 6), as well as reduction in pro-inflammatory C …
In KJD cohort (n = 9 patients), joint off-loading resulted in sustained, significant increase in SF MSC colonies' sizes and densities and a …