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53 results

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Page 1
BRET-based effector membrane translocation assay monitors GPCR-promoted and endocytosis-mediated G(q) activation at early endosomes.
Wright SC, Lukasheva V, Le Gouill C, Kobayashi H, Breton B, Mailhot-Larouche S, Blondel-Tepaz É, Antunes Vieira N, Costa-Neto C, Héroux M, Lambert NA, Parreiras-E-Silva LT, Bouvier M. Wright SC, et al. Proc Natl Acad Sci U S A. 2021 May 18;118(20):e2025846118. doi: 10.1073/pnas.2025846118. Proc Natl Acad Sci U S A. 2021. PMID: 33990469 Free PMC article.
This could have a significant impact on our understanding of how signals from GPCR-targeting drugs are propagated within the cell. However, little is known about the mechanisms that drive G protein movement and activation in subcellular compartments. Using bioluminescence …
This could have a significant impact on our understanding of how signals from GPCR-targeting drugs are propagated within the cell. However, …
Bimolecular fluorescence complementation.
Wong KA, O'Bryan JP. Wong KA, et al. J Vis Exp. 2011 Apr 15;(50):2643. doi: 10.3791/2643. J Vis Exp. 2011. PMID: 21525844 Free PMC article.
In addition, one must account for bleed through of the donor into the acceptor channel and vice versa. Unlike FRET, BiFC has little background fluorescence, little post processing of image data, does not require high overexpression, and can detect weak or transient …
In addition, one must account for bleed through of the donor into the acceptor channel and vice versa. Unlike FRET, BiFC has little b …
Sensitive and high resolution localization and tracking of membrane proteins in live cells with BRET.
Lan TH, Liu Q, Li C, Wu G, Lambert NA. Lan TH, et al. Traffic. 2012 Nov;13(11):1450-6. doi: 10.1111/j.1600-0854.2012.01401.x. Epub 2012 Aug 5. Traffic. 2012. PMID: 22816793 Free PMC article.
Here we show that bioluminescence resonance energy transfer (BRET) between membrane proteins of interest and compartment-targeted BRET partners can report subcellular location and movement of membrane proteins in live cells. ...The spatial resolution can be sufficie …
Here we show that bioluminescence resonance energy transfer (BRET) between membrane proteins of interest and compartment-targeted …
Dynamics of apelin receptor/G protein coupling in living cells.
Bai B, Jiang Y, Cai X, Chen J. Bai B, et al. Exp Cell Res. 2014 Nov 1;328(2):401-9. doi: 10.1016/j.yexcr.2014.08.035. Epub 2014 Sep 1. Exp Cell Res. 2014. PMID: 25193074
During our research on apelin receptor (APJ) signalling in living cells with BRET and FRET, we demonstrated that apelin-13 stimulation can lead to the activation of Galphai2 or Galphai3 through undergoing a molecular rearrangement rather than dissociation in HEK293 cells e …
During our research on apelin receptor (APJ) signalling in living cells with BRET and FRET, we demonstrated that apelin-13 stimulatio …
Acetylation of SUMO1 Alters Interactions with the SIMs of PML and Daxx in a Protein-Specific Manner.
Mascle XH, Gagnon C, Wahba HM, Lussier-Price M, Cappadocia L, Sakaguchi K, Omichinski JG. Mascle XH, et al. Structure. 2020 Feb 4;28(2):157-168.e5. doi: 10.1016/j.str.2019.11.019. Epub 2019 Dec 23. Structure. 2020. PMID: 31879127 Free article.
The interactions between SUMO proteins and SUMO-interacting motif (SIM) in nuclear bodies formed by the promyelocytic leukemia (PML) protein (PML-NBs) have been shown to be modulated by either phosphorylation of the SIMs or acetylation of SUMO proteins. However, little is …
The interactions between SUMO proteins and SUMO-interacting motif (SIM) in nuclear bodies formed by the promyelocytic leukemia (PML) protein …
Kinetic and Thermodynamic Insights into Agonist Interactions with the Parathyroid Hormone Receptor-1 from a New NanoBRET Assay.
Yu Z, Cary BP, Kim TW, Nguyen KD, Gardella TJ, Gellman SH. Yu Z, et al. ACS Chem Biol. 2022 Nov 18;17(11):3148-3158. doi: 10.1021/acschembio.2c00595. Epub 2022 Oct 25. ACS Chem Biol. 2022. PMID: 36282520 Free PMC article.
Affinity measurements from the BRET assay align well with results previously obtained via radioligand displacement. The BRET assay offers substantial operational benefits relative to affinity measurements involving radioactive compounds. ...
Affinity measurements from the BRET assay align well with results previously obtained via radioligand displacement. The BRET a …
Investigating protein-protein interactions in live cells using bioluminescence resonance energy transfer.
Deriziotis P, Graham SA, Estruch SB, Fisher SE. Deriziotis P, et al. J Vis Exp. 2014 May 26;(87):51438. doi: 10.3791/51438. J Vis Exp. 2014. PMID: 24893771 Free PMC article.
BRET is the non-radiative transfer of energy from a 'donor' luciferase enzyme to an 'acceptor' fluorescent protein. ...Compared to other techniques for investigating protein-protein interactions, the BRET assay is sensitive, requires little hands-on time and …
BRET is the non-radiative transfer of energy from a 'donor' luciferase enzyme to an 'acceptor' fluorescent protein. ...Compared to ot …
Rapid, single-step nucleic acid detection.
Cissell KA, Campbell S, Deo SK. Cissell KA, et al. Anal Bioanal Chem. 2008 Aug;391(7):2577-81. doi: 10.1007/s00216-008-2215-5. Epub 2008 Jun 19. Anal Bioanal Chem. 2008. PMID: 18563395
Utilizing a competitive assay, we detected the target nucleic acid by correlating the BRET signal with the amount of target present in the sample. This method allows for the detection of as little as 4 pmol (20 nM) of nucleic acid in a single-step, homogeneous forma …
Utilizing a competitive assay, we detected the target nucleic acid by correlating the BRET signal with the amount of target present i …
Organizational learning in a college of nursing: A learning history.
Lyman B, Cowan LA, Hoyt HC. Lyman B, et al. Nurse Educ Today. 2018 Feb;61:134-139. doi: 10.1016/j.nedt.2017.11.004. Epub 2017 Nov 15. Nurse Educ Today. 2018. PMID: 29197688
Organizational learning has not previously been studied in colleges of nursing, leaving college administrators and faculty little guidance as they strive to improve outcomes in their own colleges. ...
Organizational learning has not previously been studied in colleges of nursing, leaving college administrators and faculty little gui …
53 results