[Operational Performance and Microbiological Characteristics of an Iron-Salt Denitrification Reactor in Co-substrate Mode]

When iron salt is used as an autotrophic denitrification electron donor, the high iron yield generated by oxidation is easy to precipitate, resulting in "iron encrustation" on the surface of denitrifying microorganisms, which inhibits their activity and even leads to their death. In order to solve the degradation of the efficiency of the autotrophic ferric denitrification reactor caused by the "iron encrustation" coating, this paper adopted the co-substrate mode to cultivate the ferric denitrification reactor; that is, a small amount of sodium acetate was added into the water of the reactor as an organic electron donor, to realize the efficient and stable operation of the ferric denitrification reactor. The results showed that adding an appropriate amount of organic matter could make the iron salt denitrification reactor run efficiently and stably, with an efficiency of up to 0.51 kg·(m³·d)^-1, for more than 30 days. Heterotrophic bacteria could always be detected during the operation of the reactor in the co-substrate mode. Combined with the transmission electron microscopy (TEM) test results of the sludge, it was found that during the stable operation of the iron-salt denitrification reactor, heterotrophic bacteria were the main cause of iron-salt denitrification, and their unique iron-salt metabolism mode could effectively avoid the formation of iron encrustation. This study effectively solved the problem of microbial "iron encrustation" coating in the process of iron-salt denitrification, and will contribute to the development and application of autotrophic denitrification technology.