A single step reversed-phase high performance liquid chromatography separation of polar and non-polar lipids

Petter Olsson; Jan Holmbäck; Bengt Herslöf

doi:10.1016/j.chroma.2014.10.010

A single step reversed-phase high performance liquid chromatography separation of polar and non-polar lipids

J Chromatogr A. 2014 Nov 21:1369:105-15. doi: 10.1016/j.chroma.2014.10.010. Epub 2014 Oct 12.

Authors

Petter Olsson¹, Jan Holmbäck¹, Bengt Herslöf²

Affiliations

¹ Department of Analytical Chemistry, Arrhenius Laboratory, Stockholm University, SE-106 91 Stockholm, Sweden.
² Department of Analytical Chemistry, Arrhenius Laboratory, Stockholm University, SE-106 91 Stockholm, Sweden. Electronic address: bengt.herslof@anchem.su.se.

PMID: 25441077
DOI: 10.1016/j.chroma.2014.10.010

Abstract

This paper reports a simple chromatographic system to separate lipids classes as well as their molecular species. By the use of phenyl coated silica as stationary phase in combination with a simple mobile phase consisting of methanol and water, all tested lipid classes elute within 30 min. Furthermore, a method to accurately predict retention times of specific lipid components for this type of chromatography is presented. Common detection systems were used, namely evaporative light scattering detection (ELSD), charged aerosol detection (CAD), electrospray mass spectrometry (ESI-MS), and UV detection.

Keywords: Charged aerosol detector; Evaporative light scattering detector; Glycerolipids; Mass spectrometry; Phospholipids; Reversed phase liquid chromatography.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, High Pressure Liquid / instrumentation
Chromatography, High Pressure Liquid / methods
Chromatography, Reverse-Phase / instrumentation
Chromatography, Reverse-Phase / methods*
Lipids / isolation & purification*
Spectrometry, Mass, Electrospray Ionization

Substances

Lipids