Developing a Versatile Shotgun Cloning Strategy for Single-Vector-Based Multiplex Expression of Short Interfering RNAs (siRNAs) in Mammalian Cells.
Wang X, Yuan C, Huang B, Fan J, Feng Y, Li AJ, Zhang B, Lei Y, Ye Z, Zhao L, Cao D, Yang L, Wu D, Chen X, Liu B, Wagstaff W, He F, Wu X, Luo H, Zhang J, Zhang M, Haydon RC, Luu HH, Lee MJ, Moriatis Wolf J, Huang A, He TC, Zeng Z.
Wang X, et al.
ACS Synth Biol. 2019 Sep 20;8(9):2092-2105. doi: 10.1021/acssynbio.9b00203. Epub 2019 Sep 10.
ACS Synth Biol. 2019.
PMID: 31465214
Free PMC article.
Here, we developed a user-friendly system for single-vector-based multiplex siRNA expression by exploiting the unique feature of restriction endonuclease BstXI. Specifically, we engineered a BstXI-based shotgun cloning (BSG) system, which consists of three entry vec …
Here, we developed a user-friendly system for single-vector-based multiplex siRNA expression by exploiting the unique feature of restriction …