First attempts to culture keratinocytes with intention of treating burn wounds started in the 19th century (Reverdin). Fast development of tissue engineering was connected to cell culture technology improvement in the fifties. Tissue culture of isolated keratinocytes was introduced by J.G. Rheinwald and H. Green. This method was modificated many times but is still used. Autologous keratinocytes culture was applied to burn management in 1981. Primary cell cultures became more popular in burn treatment. "Cell factories" and bioreactors are used to enhance proliferation to obtain huge amounts of cells in a short time. Cells are also seeded on matrixes made from glicosamonogycans, collagen, elastin or fibrin. These products are used as a skin substitutes.