In Vivo Selection for Formate Dehydrogenases with High Efficiency and Specificity toward NADP.
Calzadiaz-Ramirez L, Calvó-Tusell C, Stoffel GMM, Lindner SN, Osuna S, Erb TJ, Garcia-Borràs M, Bar-Even A, Acevedo-Rocha CG.
Calzadiaz-Ramirez L, et al.
ACS Catal. 2020 Jul 17;10(14):7512-7525. doi: 10.1021/acscatal.0c01487. Epub 2020 Jun 8.
ACS Catal. 2020.
PMID: 32733773
Free PMC article.
We introduce this library into an E. coli strain that cannot produce NADPH. By selecting for growth with formate as the sole NADPH source, we isolate several enzyme variants that support efficient NADPH regeneration. ...
We introduce this library into an E. coli strain that cannot produce NADPH. By selecting for growth with formate as the sole NADPH so …