The potential of nuclear magnetic resonance (NMR) in metabolomics and lipidomics of microalgae- a review

Bhawna Bisht; Vinod Kumar; Prateek Gururani; Mahipal Singh Tomar; Manisha Nanda; Makhail S Vlaskin; Sanjay Kumar; Anna Kurbatova

doi:10.1016/j.abb.2021.108987

The potential of nuclear magnetic resonance (NMR) in metabolomics and lipidomics of microalgae- a review

Arch Biochem Biophys. 2021 Oct 15:710:108987. doi: 10.1016/j.abb.2021.108987. Epub 2021 Jul 11.

Authors

Bhawna Bisht¹, Vinod Kumar², Prateek Gururani³, Mahipal Singh Tomar⁴, Manisha Nanda⁵, Makhail S Vlaskin⁶, Sanjay Kumar⁷, Anna Kurbatova⁸

Affiliations

¹ Department of Post-Harvest Process and Food Engineering, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India.
² Department of Life Sciences, Graphic Era (Deemed to Be University), Dehradun, Uttarakhand 248002, India; Peoples' Friendship University of Russia (RUDN University), Moscow, 117198, Russian Federation. Electronic address: kumar.ls@geu.ac.in.
³ Department of Biotechnology, Graphic Era (Deemed to Be University), Dehradun, Uttarakhand 248002, India.
⁴ Department of Food Process Engineering, National Institute of Technology, Rourkela, India.
⁵ Department of Biotechnology, Dolphin (PG) Institute of Biomedical and Natural Sciences, Dehradun, Uttarakhand 248007, India.
⁶ Joint Institute for High Temperatures of the Russian Academy of Sciences, 13/2 Izhorskaya St, Moscow 125412, Russian Federation.
⁷ Department of Life Sciences, Graphic Era (Deemed to Be University), Dehradun, Uttarakhand 248002, India.
⁸ Department of Environmental Monitoring and Forecasting, Ecological Faculty, RUDN University, Moscow, 117198, Russia.

PMID: 34260946
DOI: 10.1016/j.abb.2021.108987

Abstract

Microalgae biotechnology has made it possible to derive secondary bioactive metabolites from microalgae strains that have opened up their entire potential to uncover a wide range of novel metabolic capabilities and turn these into bio-products for the development of sustainable bio-refineries. Nuclear Magnetic Resonance Technology (NMR) has been one of the most successful and functional research technology over the past two decades to analyse the composition, structure and functionality of distinct metabolites in the different microalgae strains. This technology offers qualitative as well as quantitative knowledge about the endogenous metabolites and lipids of low molecular mass to offer a good picture of the physiological state of biological samples in metabolomics and lipidomics studies. Henceforth, this review is aimed at introducing the metabolomics and lipidomics studies into the field of NMR technology and also highlights the protocols for the isolation and metabolic measurements of metabolites from microalgae that should be redirected to resource recovery and value-added products with a systematic and holistic approach for scalability or sustainability.

Keywords: Biotechnology; Lipidomics; Metabolomics; Microalgae; NMR; Sustainable.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Aquaculture
Biomass
Biotechnology / methods
Food Chain
Hydrogen
Lipidomics / methods*
Lipids / analysis
Lipids / chemistry
Metabolomics / methods*
Microalgae / chemistry*
Microalgae / metabolism*
Proton Magnetic Resonance Spectroscopy / methods*
Solvents

Substances

Lipids
Solvents
Hydrogen