Eco-friendly green synthesis of clove buds extract functionalized silver nanoparticles and evaluation of antibacterial and antidiatom activity

Muhammad Nazim Lakhan; Rongrong Chen; Altaf Hussain Shar; Kishore Chand; Ahmer Hussain Shah; Mukhtiar Ahmed; Irfan Ali; Rizwan Ahmed; Jingyuan Liu; Kazunobu Takahashi; Jun Wang

doi:10.1016/j.mimet.2020.105934

Eco-friendly green synthesis of clove buds extract functionalized silver nanoparticles and evaluation of antibacterial and antidiatom activity

J Microbiol Methods. 2020 Jun:173:105934. doi: 10.1016/j.mimet.2020.105934. Epub 2020 Apr 21.

Authors

Affiliations

¹ Key Laboratory of Superlight Material and Surface Technology, Ministry of Education, College of Materials Science and Chemical Engineering, Harbin Engineering University, PR China.
² Key Laboratory of Superlight Material and Surface Technology, Ministry of Education, College of Materials Science and Chemical Engineering, Harbin Engineering University, PR China. Electronic address: chenrongrong@hrbeu.edu.cn.
³ Department of Textile Engineering, Balochistan University of Information Technology, Engineering and Management Science, Quetta, Pakistan.
⁴ College of Chemical Engineering, Beijing University of Chemical Technology, PR China.
⁵ School of Chemical Engineering, Dalian University of Technology, PR China.
⁶ Key Laboratory of Superlight Material and Surface Technology, Ministry of Education, College of Materials Science and Chemical Engineering, Harbin Engineering University, PR China. Electronic address: zhqw1888@sohu.com.

PMID: 32325159
DOI: 10.1016/j.mimet.2020.105934

Abstract

Biological fouling has caused a lot of concern in marine industries due to the attachment of microorganisms on marine surfaces. Silver nanoparticles (AgNPs) have a great potential to inhibit and hold strong toxicity against microorganisms on artificial surfaces immersed in seawater. In this study, AgNPs are synthesized using extract of clove buds (CE) plant as a reducing and stabilizing agent by biological synthesis method. The obtained CE-AgNPs product was characterized by using different techniques. Ultraviolet-visible spectroscopy (UV-Vis) results confirmed the formation of CE-AgNPs with its surface plasmon resonance peak range. Fourier-transform infrared spectroscopy (FTIR) study showed the formation of functional groups responsible for the reduction of Ag⁺ into Ag^o. X-Ray Diffraction (XRD) results revealed face-centered cubic (fcc) silver crystals having four different diffraction peaks at 38.08, 44.21, 64.42 and 77.32 with corresponding lattice plane value recorded at (111), (200), (220) and (311), respectively. Structural characterization using scanning electron microscopy equipped with energy dispersive X-Ray Analyzer (SEM-EDX), Transmission electron microscopy (TEM) and atomic force microscopy (AFM) also confirmed the successful formation of CE-AgNPs with fcc structure. The histogram of particle size distribution through TEM image showed an average size of 9.42 nm of the synthesized product. Finally, the antibacterial and antidiatom activity of the synthesized product was analyzed. The CE-AgNPs synthesized using CE possesses good inhibitory activity against the marine bacterium community and Nitzschia closterium diatom. These results indicate that CE-AgNPs can be used as a novel material for antibacterial and antidiatoms means to inhibit the biofouling on marine surfaces.

Keywords: Antibacterial activity; Antidiatom activity; Biological synthesis; Marine biofouling; Silver nanoparticles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology*
China
Green Chemistry Technology / methods*
Metal Nanoparticles / chemistry*
Microbial Sensitivity Tests
Particle Size
Plant Extracts / chemistry*
Silver / chemistry
Spectroscopy, Fourier Transform Infrared / methods
Surface Plasmon Resonance / methods
Syzygium / chemistry*
X-Ray Diffraction

Substances

Anti-Bacterial Agents
Plant Extracts
Silver