Glutamate Supply Reactivates Ovarian Function while Increases Serum Insulin and Triiodothyronine Concentrations in Criollo x Saanen-Alpine Yearlings' Goats during the Anestrous Season

Animals (Basel). 2020 Feb 2;10(2):234. doi: 10.3390/ani10020234.

Abstract

The possible effect of glutamate supplementation upon ovarian reactivation and serum concentrations of insulin (INS) and triiodothyronine (T3) in anestrous yearling goats was evaluated. Goats (n = 32, 12 mo., 26° North, 1117 m) with a similar live weight (LW) and body condition score (BCS) were blood sampled twice per week for two weeks (2 × 1 week × 2 weeks) to confirm the anestrus status (<1 ng P4/mL; RIA). Thereafter, goats were randomly assigned to either 1) Glutamate (GLUT; n = 16, LW = 27.1 ± 1.09 kg, 3.5 ± 0.18 units, IV-supplemented with 7 mg of glutamate kg-1 LW), or 2) Control (CONT; n = 16; LW = 29.2 ± 1.09 kg; BCS = 3.5 ± 0.18, IV saline). During the treatment period, 16 goats (eight/group) were blood sampled twice per week for six weeks. Such serum samples (2 × 1 week × 6 weeks) were quantified by their P4 content to evaluate the ovarian-luteal activity, whereas a sample subset (1 × 1 week × 6 weeks) was used to quantify their INS & T3 content to evaluate their metabolic status. Neither LW (28.19 kg; p > 0.05) nor BCS (3.51 units; p > 0.05) differed between treatments. Goats depicting ovarian reactivation favored the GLUT group (50 vs. 12.5%; p < 0.05). Neither INS (1.72 ± 0.15 ng mL-1) nor T3 (2.32 ± 0.11 ng mL-1) differed between treatments, yet a treatment x time interaction regarding INS & T3 concentration across time favored (p < 0.05) the GLUT group. The results unveil exogenous glutamate as an interesting modulator not only of ovarian reactivation, but of metabolic hormone synthesis.

Keywords: anestrous; glutamate supply; goats; metabolic hormones; ovarian reactivation.