Search for Promising Strains of Probiotic Microbiota Isolated from Different Biotopes of Healthy Cats for Use in the Control of Surgical Infections

Pavel Rudenko; Yuriy Vatnikov; Nadezhda Sachivkina; Andrei Rudenko; Evgeny Kulikov; Vladimir Lutsay; Elena Notina; Irina Bykova; Aleksander Petrov; Stanislav Drukovskiy; Ifarajimi Rapheal Olabode

doi:10.3390/pathogens10060667

Search for Promising Strains of Probiotic Microbiota Isolated from Different Biotopes of Healthy Cats for Use in the Control of Surgical Infections

Pathogens. 2021 May 28;10(6):667. doi: 10.3390/pathogens10060667.

Authors

Affiliations

¹ Biological Testing Laboratory, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences (BIBCh RAS), 142290 Pushchino, Russia.
² Department of Veterinary Medicine, Peoples' Friendship University of Russia (RUDN University), 117198 Moscow, Russia.
³ Department of Microbiology and Virology, Peoples' Friendship University of Russia (RUDN University), 117198 Moscow, Russia.
⁴ Department of Veterinary Medicine, Moscow State University of Food Production, 125080 Moscow, Russia.
⁵ Department of Foreign Languages, Peoples' Friendship University of Russia (RUDN University), 117198 Moscow, Russia.

Abstract

Despite the introduction of modern methods of treatment, the creation of new generations of antibacterial agents, and the constant improvement of aseptic and antiseptic methods, the treatment of purulent-inflammatory processes remains one of the most complex and urgent problems in veterinary practice. The article presents the results of the isolation of indigenous microbiota from various biotopes of healthy cats, as well as the study of their biological marker properties for the selection of the most optimal strains in probiotic medicines for the control of surgical infections. It was demonstrated that isolated cultures of bifidobacteria and lactobacilli, which we isolated, revealed high sensitivity to antibiotics of the β-lactam group (excepting L. acidophilus No. 24, L. plantarum "Victoria" No. 22, L. rhamnosus No. 5, L. rhamnosus No. 20, and L. rhamnosus No. 26, which showed a significant variability in sensitivity to antibacterial drugs of this group, indicating the great potential of these microorganisms) and resistance to aminoglycosides, lincosamides, and fluoroquinolones (with the exception of gatifloxacin, which showed high efficiency in relation to all lactic acid microorganisms). The adhesive properties of the isolated lactobacteria and bifidobacteria were variable, even within the same species. It was found that the B. adolescentis No. 23 strain of the Bifidobacterium genus, as well as the L. plantarum No. 8, L. plantarum "Victoria" No. 22, L. rhamnosus No. 6, L. rhamnosus No. 26, L. acidophilus No. 12, and L. acidophilus No. 24 strains of the Lactobacillus genus had the highest adhesive activity. Thus, when conducting a detailed analysis of the biological marker properties of candidate cultures (determining their sensitivity to antimicrobial agents, studying the adhesive properties, and antagonistic activity in relation to causative agents of surgical infection in cats), it was found that the most promising are L. plantarum "Victoria" No. 22, L. rhamnosus No. 26, and L. acidophilus No. 24.

Keywords: adhesion; antagonism; antibiotics; biotope; cats; microbiota; probiotics; surgical infection.