The GABA(A)ρ receptors in hippocampal spontaneous activity and their distribution in hippocampus, amygdala and visual cortex

Neurosci Lett. 2011 Aug 1;500(1):20-5. doi: 10.1016/j.neulet.2011.05.235. Epub 2011 Jun 12.

Abstract

A bicuculline-resistant and TPMPA-sensitive GABAergic component was identified in hippocampal neurons in culture and in acute isolated brain slices. In both preparations, total GABAergic activity showed two inactivation kinetics: fast and slow. RT-PCR, in situ hybridization (ISH) and immunohistochemistry detected expression of GABAρ subunits. Immunogold and electron microscopy indicated that the receptors are mostly extrasynaptic. In addition, by RT-PCR and immunofluorescence we found GABAρ present in amygdala and visual cortex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amygdala / physiology*
  • Animals
  • Embryo, Nonmammalian / cytology
  • Hippocampus / cytology
  • Hippocampus / physiology*
  • Immunohistochemistry
  • In Situ Hybridization
  • In Vitro Techniques
  • Mice
  • Mice, Inbred C57BL
  • Miniature Postsynaptic Potentials
  • Neurons / physiology*
  • Patch-Clamp Techniques
  • Protein Subunits / physiology
  • Pyramidal Cells / physiology
  • Receptors, GABA-A / physiology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Synapses / physiology
  • Visual Cortex / physiology*

Substances

  • Protein Subunits
  • Receptors, GABA-A