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Identification and removal of unexpected proliferative off-target cells emerging after iPSC-derived pancreatic islet cell implantation.
Hiyoshi H, Sakuma K, Asano S, Napier SC, Konagaya S, Mochida T, Ueno H, Watanabe T, Kassai Y, Matsumoto H, Ito R, Toyoda T. Hiyoshi H, et al. Among authors: watanabe t. Proc Natl Acad Sci U S A. 2024 Apr 16;121(16):e2320883121. doi: 10.1073/pnas.2320883121. Epub 2024 Apr 10. Proc Natl Acad Sci U S A. 2024. PMID: 38598342 Free PMC article.
CDK8/19 inhibition plays an important role in pancreatic β-cell induction from human iPSCs.
Sakuma K, Tsubooka-Yamazoe N, Hashimoto K, Sakai N, Asano S, Watanabe-Matsumoto S, Watanabe T, Saito B, Matsumoto H, Ueno H, Ito R, Toyoda T. Sakuma K, et al. Among authors: watanabe t, watanabe matsumoto s. Stem Cell Res Ther. 2023 Jan 5;14(1):1. doi: 10.1186/s13287-022-03220-4. Stem Cell Res Ther. 2023. PMID: 36600289 Free PMC article.
Multisite studies for validation and improvement of a highly efficient culture assay for detection of undifferentiated human pluripotent stem cells intermingled in cell therapy products.
Watanabe T, Yasuda S, Kusakawa S, Kuroda T, Futamura M, Ogawa M, Mochizuki H, Kikkawa E, Furukawa H, Nagaoka M, Sato Y. Watanabe T, et al. Cytotherapy. 2021 Feb;23(2):176-183. doi: 10.1016/j.jcyt.2020.07.009. Epub 2020 Sep 22. Cytotherapy. 2021. PMID: 32978066 Free article.
Protocol improvement and multisite validation of a digital soft agar colony formation assay for tumorigenic transformed cells intermingled in cell therapy products.
Bando K, Kusakawa S, Adachi H, Yamamoto M, Iwata M, Kitanaka A, Ogimura E, Osada T, Tamura M, Terai O, Watanabe T, Yoda T, Yotsumoto T, Zaizen K, Sato Y. Bando K, et al. Among authors: watanabe t. Cytotherapy. 2024 Mar 13:S1465-3249(24)00094-X. doi: 10.1016/j.jcyt.2024.03.005. Online ahead of print. Cytotherapy. 2024. PMID: 38556961 Free article.
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