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Fusion crystallization reveals the behavior of both the 1TEL crystallization chaperone and the TNK1 UBA domain.
Structure. 2023 Dec 7;31(12):1589-1603.e6. doi: 10.1016/j.str.2023.09.001. Epub 2023 Sep 29.
Structure. 2023.
PMID: 37776857
Free article.
Increasing the bulk of the 1TEL-target linker and retaining the 10×His tag in a 1TEL-CMG2-vWa construct improves crystal order and diffraction limits.
Gajjar PL, Pedroza Romo MJ, Litchfield CM, Callahan M, Redd N, Nawarathnage S, Soleimani S, Averett J, Wilson E, Lewis A, Stewart C, Tseng YJ, Doukov T, Lebedev A, Moody JD.
Gajjar PL, et al. Among authors: pedroza romo mj.
Acta Crystallogr D Struct Biol. 2023 Oct 1;79(Pt 10):925-943. doi: 10.1107/S2059798323007246. Epub 2023 Sep 25.
Acta Crystallogr D Struct Biol. 2023.
PMID: 37747038
Free PMC article.
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Crystals of TELSAM-target protein fusions that exhibit minimal crystal contacts and lack direct inter-TELSAM contacts.
Nawarathnage S, Soleimani S, Mathis MH, Bezzant BD, Ramírez DT, Gajjar P, Bunn DR, Stewart C, Smith T, Pedroza Romo MJ, Brown S, Doukov T, Moody JD.
Nawarathnage S, et al. Among authors: pedroza romo mj.
Open Biol. 2022 Mar;12(3):210271. doi: 10.1098/rsob.210271. Epub 2022 Mar 2.
Open Biol. 2022.
PMID: 35232248
Free PMC article.
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