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Increasing the bulk of the 1TEL-target linker and retaining the 10×His tag in a 1TEL-CMG2-vWa construct improves crystal order and diffraction limits.
Acta Crystallogr D Struct Biol. 2023 Oct 1;79(Pt 10):925-943. doi: 10.1107/S2059798323007246. Epub 2023 Sep 25.
Acta Crystallogr D Struct Biol. 2023.
PMID: 37747038
Free PMC article.
Decreasing the flexibility of the TELSAM-target protein linker and omitting the cleavable fusion tag improves crystal order and diffraction limits.
Gajjar PL, Romo MJP, Litchfield CM, Callahan M, Redd N, Nawarathnage S, Soleimani S, Averett J, Wilson E, Lewis A, Stewart C, Tseng YJ, Doukov T, Lebedev A, Moody JD.
Gajjar PL, et al. Among authors: litchfield cm.
bioRxiv [Preprint]. 2023 May 15:2023.05.12.540586. doi: 10.1101/2023.05.12.540586.
bioRxiv. 2023.
PMID: 37293010
Free PMC article.
Preprint.
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