Isolation and characterization of soluble human full-length TDP-43 associated with neurodegeneration.
Vivoli Vega M, Nigro A, Luti S, Capitini C, Fani G, Gonnelli L, Boscaro F, Chiti F.
Vivoli Vega M, et al. Among authors: luti s.
FASEB J. 2019 Oct;33(10):10780-10793. doi: 10.1096/fj.201900474R. Epub 2019 Jul 9.
FASEB J. 2019.
PMID: 31287959
Using a novel protocol we have achieved the purification of the full-length TDP-43, with both a short pectate lyase B tag and a glutathione S-transferase tag, which consisted in its expression in bacteria, solubilization from inclusion bodies, purification under denaturing …
Using a novel protocol we have achieved the purification of the full-length TDP-43, with both a short pectate lyase B tag and a glutathione …