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Oocyte electroporation prior to in vitro fertilization is an efficient method to generate single, double, and multiple knockout porcine embryos of interest in biomedicine and animal production.
Navarro-Serna S, Piñeiro-Silva C, Fernández-Martín I, Dehesa-Etxebeste M, López de Munain A, Gadea J. Navarro-Serna S, et al. Theriogenology. 2024 Apr 1;218:111-118. doi: 10.1016/j.theriogenology.2024.01.040. Epub 2024 Feb 1. Theriogenology. 2024. PMID: 38320372 Free article.
One-Step In Vitro Generation of ETV2-Null Pig Embryos.
Moya-Jódar M, Coppiello G, Rodríguez-Madoz JR, Abizanda G, Barlabé P, Vilas-Zornoza A, Ullate-Agote A, Luongo C, Rodríguez-Tobón E, Navarro-Serna S, París-Oller E, Oficialdegui M, Carvajal-Vergara X, Ordovás L, Prósper F, García-Vázquez FA, Aranguren XL. Moya-Jódar M, et al. Among authors: navarro serna s. Animals (Basel). 2022 Jul 18;12(14):1829. doi: 10.3390/ani12141829. Animals (Basel). 2022. PMID: 35883376 Free PMC article.
Generation of Nonmosaic, Two-Pore Channel 2 Biallelic Knockout Pigs in One Generation by CRISPR-Cas9 Microinjection Before Oocyte Insemination.
Navarro-Serna S, Hachem A, Canha-Gouveia A, Hanbashi A, Garrappa G, Lopes JS, París-Oller E, Sarrías-Gil L, Flores-Flores C, Bassett A, Sánchez R, Bermejo-Álvarez P, Matás C, Romar R, Parrington J, Gadea J. Navarro-Serna S, et al. CRISPR J. 2021 Feb;4(1):132-146. doi: 10.1089/crispr.2020.0078. CRISPR J. 2021. PMID: 33616447
13 results