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Proteinase K treatment in absence of RNA isolation classical procedures is a quick and cheaper alternative for SARS-CoV-2 molecular detection.
Mallmann L, Hermann BS, Schallenberger K, Demoliner M, Eisen AKA, Heldt FH, Gularte JS, Hansen AW, de Almeida PR, Weber MN, Spilki FR, Fleck JD. Mallmann L, et al. Among authors: schallenberger k. J Virol Methods. 2021 Jul;293:114131. doi: 10.1016/j.jviromet.2021.114131. Epub 2021 Mar 30. J Virol Methods. 2021. PMID: 33798606 Free PMC article.
Viral isolation allows characterization of early samples of SARS-CoV-2 lineage B1.1.33 with unique mutations (S: H655Y and T63N) circulating in Southern Brazil in 2020.
Gularte JS, da Silva MS, Filippi M, Demoliner M, Schallenberger K, Hansen AW, de Abreu Góes Pereira VM, Heldt FH, Girardi V, Weber MN, de Almeida PR, Abbadi BL, Dornelles M, Bizarro CV, Machado P, Basso LA, Dellagostin OA, Fleck JD, Spilki FR. Gularte JS, et al. Among authors: schallenberger k. Braz J Microbiol. 2022 Sep;53(3):1313-1319. doi: 10.1007/s42770-022-00789-z. Epub 2022 Jul 1. Braz J Microbiol. 2022. PMID: 35778549 Free PMC article.
Pervasive transmission of E484K and emergence of VUI-NP13L with evidence of SARS-CoV-2 co-infection events by two different lineages in Rio Grande do Sul, Brazil.
Francisco RDS Jr, Benites LF, Lamarca AP, de Almeida LGP, Hansen AW, Gularte JS, Demoliner M, Gerber AL, de C Guimarães AP, Antunes AKE, Heldt FH, Mallmann L, Hermann B, Ziulkoski AL, Goes V, Schallenberger K, Fillipi M, Pereira F, Weber MN, de Almeida PR, Fleck JD, Vasconcelos ATR, Spilki FR. Francisco RDS Jr, et al. Among authors: schallenberger k. Virus Res. 2021 Apr 15;296:198345. doi: 10.1016/j.virusres.2021.198345. Epub 2021 Feb 22. Virus Res. 2021. PMID: 33631222 Free PMC article.