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Development of a Cas12a-Based Genome Editing Tool for Moderate Thermophiles.
Mohanraju P, Mougiakos I, Albers J, Mabuchi M, Fuchs RT, Curcuru JL, van Kranenburg R, Robb GB, van der Oost J. Mohanraju P, et al. Among authors: mabuchi m. CRISPR J. 2021 Feb;4(1):82-91. doi: 10.1089/crispr.2020.0086. Epub 2021 Feb 4. CRISPR J. 2021. PMID: 33538626 Free PMC article.
Staphylococcus aureus Cas9 is a multiple-turnover enzyme.
Yourik P, Fuchs RT, Mabuchi M, Curcuru JL, Robb GB. Yourik P, et al. Among authors: mabuchi m. RNA. 2019 Jan;25(1):35-44. doi: 10.1261/rna.067355.118. Epub 2018 Oct 22. RNA. 2019. PMID: 30348755 Free PMC article.
A catalogue of biochemically diverse CRISPR-Cas9 orthologs.
Gasiunas G, Young JK, Karvelis T, Kazlauskas D, Urbaitis T, Jasnauskaite M, Grusyte MM, Paulraj S, Wang PH, Hou Z, Dooley SK, Cigan M, Alarcon C, Chilcoat ND, Bigelyte G, Curcuru JL, Mabuchi M, Sun Z, Fuchs RT, Schildkraut E, Weigele PR, Jack WE, Robb GB, Venclovas Č, Siksnys V. Gasiunas G, et al. Among authors: mabuchi m. Nat Commun. 2020 Nov 2;11(1):5512. doi: 10.1038/s41467-020-19344-1. Nat Commun. 2020. PMID: 33139742 Free PMC article.
Chemically modified guide RNAs enhance CRISPR-Cas13 knockdown in human cells.
Méndez-Mancilla A, Wessels HH, Legut M, Kadina A, Mabuchi M, Walker J, Robb GB, Holden K, Sanjana NE. Méndez-Mancilla A, et al. Among authors: mabuchi m. Cell Chem Biol. 2022 Feb 17;29(2):321-327.e4. doi: 10.1016/j.chembiol.2021.07.011. Epub 2021 Aug 2. Cell Chem Biol. 2022. PMID: 34343484 Free PMC article.
Characterization of Cme and Yme thermostable Cas12a orthologs.
Fuchs RT, Curcuru JL, Mabuchi M, Noireterre A, Weigele PR, Sun Z, Robb GB. Fuchs RT, et al. Among authors: mabuchi m. Commun Biol. 2022 Apr 6;5(1):325. doi: 10.1038/s42003-022-03275-2. Commun Biol. 2022. PMID: 35388146 Free PMC article.
Precise DNA cleavage using CRISPR-SpRYgests.
Christie KA, Guo JA, Silverstein RA, Doll RM, Mabuchi M, Stutzman HE, Lin J, Ma L, Walton RT, Pinello L, Robb GB, Kleinstiver BP. Christie KA, et al. Among authors: mabuchi m. Nat Biotechnol. 2023 Mar;41(3):409-416. doi: 10.1038/s41587-022-01492-y. Epub 2022 Oct 6. Nat Biotechnol. 2023. PMID: 36203014 Free PMC article.
A new family of CRISPR-type V nucleases with C-rich PAM recognition.
Urbaitis T, Gasiunas G, Young JK, Hou Z, Paulraj S, Godliauskaite E, Juskeviciene MM, Stitilyte M, Jasnauskaite M, Mabuchi M, Robb GB, Siksnys V. Urbaitis T, et al. Among authors: mabuchi m. EMBO Rep. 2022 Dec 6;23(12):e55481. doi: 10.15252/embr.202255481. Epub 2022 Oct 21. EMBO Rep. 2022. PMID: 36268581 Free PMC article.
144 results