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A strategy based on thermal flexibility to design triosephosphate isomerase proteins with increased or decreased kinetic stability.
Biochem Biophys Res Commun. 2018 Sep 18;503(4):3017-3022. doi: 10.1016/j.bbrc.2018.08.087. Epub 2018 Aug 22.
Biochem Biophys Res Commun. 2018.
PMID: 30143261
Three unrelated and unexpected amino acids determine the susceptibility of the interface cysteine to a sulfhydryl reagent in the triosephosphate isomerases of two trypanosomes.
Díaz-Mazariegos S, Cabrera N, Perez-Montfort R.
Díaz-Mazariegos S, et al.
PLoS One. 2018 Jan 17;13(1):e0189525. doi: 10.1371/journal.pone.0189525. eCollection 2018.
PLoS One. 2018.
PMID: 29342154
Free PMC article.
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Identification of amino acids that account for long-range interactions in two triosephosphate isomerases from pathogenic trypanosomes.
García-Torres I, Cabrera N, Torres-Larios A, Rodríguez-Bolaños M, Díaz-Mazariegos S, Gómez-Puyou A, Perez-Montfort R.
García-Torres I, et al. Among authors: diaz mazariegos s.
PLoS One. 2011 Apr 18;6(4):e18791. doi: 10.1371/journal.pone.0018791.
PLoS One. 2011.
PMID: 21533154
Free PMC article.
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Potent and Selective Inhibitors of Trypanosoma cruzi Triosephosphate Isomerase with Concomitant Inhibition of Cruzipain: Inhibition of Parasite Growth through Multitarget Activity.
Aguilera E, Varela J, Birriel E, Serna E, Torres S, Yaluff G, de Bilbao NV, Aguirre-López B, Cabrera N, Díaz Mazariegos S, de Gómez-Puyou MT, Gómez-Puyou A, Pérez-Montfort R, Minini L, Merlino A, Cerecetto H, González M, Alvarez G.
Aguilera E, et al. Among authors: diaz mazariegos s.
ChemMedChem. 2016 Jun 20;11(12):1328-38. doi: 10.1002/cmdc.201500385. Epub 2015 Oct 23.
ChemMedChem. 2016.
PMID: 26492824
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