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DNA immunization combined with scFv phage display identifies antagonistic GCGR specific antibodies and reveals new epitopes on the small extracellular loops.
van der Woning B, De Boeck G, Blanchetot C, Bobkov V, Klarenbeek A, Saunders M, Waelbroeck M, Laeremans T, Steyaert J, Hultberg A, De Haard H. van der Woning B, et al. Among authors: steyaert j. MAbs. 2016 Aug-Sep;8(6):1126-35. doi: 10.1080/19420862.2016.1189050. Epub 2016 May 21. MAbs. 2016. PMID: 27211075 Free PMC article.
A general protocol for the generation of Nanobodies for structural biology.
Pardon E, Laeremans T, Triest S, Rasmussen SG, Wohlkönig A, Ruf A, Muyldermans S, Hol WG, Kobilka BK, Steyaert J. Pardon E, et al. Among authors: steyaert j. Nat Protoc. 2014 Mar;9(3):674-93. doi: 10.1038/nprot.2014.039. Epub 2014 Feb 27. Nat Protoc. 2014. PMID: 24577359 Free PMC article.
Propagation of conformational changes during μ-opioid receptor activation.
Sounier R, Mas C, Steyaert J, Laeremans T, Manglik A, Huang W, Kobilka BK, Déméné H, Granier S. Sounier R, et al. Among authors: steyaert j. Nature. 2015 Aug 20;524(7565):375-8. doi: 10.1038/nature14680. Epub 2015 Aug 5. Nature. 2015. PMID: 26245377 Free PMC article.
Structural insights into µ-opioid receptor activation.
Huang W, Manglik A, Venkatakrishnan AJ, Laeremans T, Feinberg EN, Sanborn AL, Kato HE, Livingston KE, Thorsen TS, Kling RC, Granier S, Gmeiner P, Husbands SM, Traynor JR, Weis WI, Steyaert J, Dror RO, Kobilka BK. Huang W, et al. Among authors: steyaert j. Nature. 2015 Aug 20;524(7565):315-21. doi: 10.1038/nature14886. Epub 2015 Aug 5. Nature. 2015. PMID: 26245379 Free PMC article.
Crystal Structures of the Human Doublecortin C- and N-terminal Domains in Complex with Specific Antibodies.
Burger D, Stihle M, Sharma A, Di Lello P, Benz J, D'Arcy B, Debulpaep M, Fry D, Huber W, Kremer T, Laeremans T, Matile H, Ross A, Rufer AC, Schoch G, Steinmetz MO, Steyaert J, Rudolph MG, Thoma R, Ruf A. Burger D, et al. Among authors: steyaert j. J Biol Chem. 2016 Jul 29;291(31):16292-306. doi: 10.1074/jbc.M116.726547. Epub 2016 May 10. J Biol Chem. 2016. PMID: 27226599 Free PMC article.
Nanobody-Enabled Reverse Pharmacology on G-Protein-Coupled Receptors.
Pardon E, Betti C, Laeremans T, Chevillard F, Guillemyn K, Kolb P, Ballet S, Steyaert J. Pardon E, et al. Among authors: steyaert j. Angew Chem Int Ed Engl. 2018 May 4;57(19):5292-5295. doi: 10.1002/anie.201712581. Epub 2018 Mar 30. Angew Chem Int Ed Engl. 2018. PMID: 29469969
Development by Genetic Immunization of Monovalent Antibodies Against Human Vasoactive Intestinal Peptide Receptor 1 (VPAC1), New Innovative, and Versatile Tools to Study VPAC1 Receptor Function.
Peyrassol X, Laeremans T, Lahura V, Debulpaep M, El Hassan H, Steyaert J, Parmentier M, Langer I. Peyrassol X, et al. Among authors: steyaert j. Front Endocrinol (Lausanne). 2018 Apr 5;9:153. doi: 10.3389/fendo.2018.00153. eCollection 2018. Front Endocrinol (Lausanne). 2018. PMID: 29674997 Free PMC article.
425 results