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The use of Ni-nitrilotriacetic acid agarose for estimation of affinities of hexahistidine-tagged Fab to single-stranded DNA.
Komissarov AA, Marchbank MT, Deutscher SL. Komissarov AA, et al. Anal Biochem. 1997 Apr 5;247(1):123-9. doi: 10.1006/abio.1997.2051. Anal Biochem. 1997. PMID: 9126381
High stability of the immobilized complex (half-life of 4 h) and low nonspecific binding of (32P](dT)15 allowed for a rapid estimation of the dissociation constant (Kd) and was found to be approximately 130 nM. ...A. Komissarov, M. J. Calcutt, M. T. Marchbank …
High stability of the immobilized complex (half-life of 4 h) and low nonspecific binding of (32P](dT)15 allowed for a rapid estimatio …
Site-specific mutagenesis of a recombinant anti-single-stranded DNA Fab. Role of heavy chain complementarity-determining region 3 residues in antigen interaction.
Komissarov AA, Marchbank MT, Calcutt MJ, Quinn TP, Deutscher SL. Komissarov AA, et al. J Biol Chem. 1997 Oct 24;272(43):26864-70. doi: 10.1074/jbc.272.43.26864. J Biol Chem. 1997. PMID: 9341118 Free article.
The heavy chain complementarity-determining region 3 (HCDR3) of the anti-oligo(dT) recombinant antibody fragment, DNA-1, contributes significantly to antigen binding (Komissarov, A. A., Calcutt, M. J., Marchbank, M. T., Peletskaya, E. ...The highly conserved …
The heavy chain complementarity-determining region 3 (HCDR3) of the anti-oligo(dT) recombinant antibody fragment, DNA-1, contributes signifi …
Equilibrium binding studies of recombinant anti-single-stranded DNA Fab. Role of heavy chain complementarity-determining regions.
Komissarov AA, Calcutt MJ, Marchbank MT, Peletskaya EN, Deutsher SL. Komissarov AA, et al. J Biol Chem. 1996 May 24;271(21):12241-6. doi: 10.1074/jbc.271.21.12241. J Biol Chem. 1996. PMID: 8647821 Free article.
A detailed analysis of the involvement of the individual heavy chain (H) complementarity-determining regions (CDR) ensued using previously constructed HCDR transplantation mutants between DNA-1 and low affinity ssDNA-binding Fab (D5), a Fab that binds poorly to DNA …
A detailed analysis of the involvement of the individual heavy chain (H) complementarity-determining regions (CDR) ensued using previ …
245 results