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Amides are excellent mimics of phosphate internucleoside linkages and are well tolerated in short interfering RNAs.
Nucleic Acids Res. 2014 Jun;42(10):6542-51. doi: 10.1093/nar/gku235. Epub 2014 May 9.
Nucleic Acids Res. 2014.
PMID: 24813446
Free PMC article.
Synthesis, biophysical studies and RNA interference activity of RNA having three consecutive amide linkages.
Tanui P, Kennedy SD, Lunstad BD, Haas A, Leake D, Rozners E.
Tanui P, et al. Among authors: lunstad bd.
Org Biomol Chem. 2014 Feb 28;12(8):1207-10. doi: 10.1039/c3ob42532k.
Org Biomol Chem. 2014.
PMID: 24435630
Free PMC article.
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Biochemical properties of phosphonoacetate and thiophosphonoacetate oligodeoxyribonucleotides.
Sheehan D, Lunstad B, Yamada CM, Stell BG, Caruthers MH, Dellinger DJ.
Sheehan D, et al.
Nucleic Acids Res. 2003 Jul 15;31(14):4109-18. doi: 10.1093/nar/gkg439.
Nucleic Acids Res. 2003.
PMID: 12853628
Free PMC article.
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Improving CRISPR-Cas specificity with chemical modifications in single-guide RNAs.
Ryan DE, Taussig D, Steinfeld I, Phadnis SM, Lunstad BD, Singh M, Vuong X, Okochi KD, McCaffrey R, Olesiak M, Roy S, Yung CW, Curry B, Sampson JR, Bruhn L, Dellinger DJ.
Ryan DE, et al. Among authors: lunstad bd.
Nucleic Acids Res. 2018 Jan 25;46(2):792-803. doi: 10.1093/nar/gkx1199.
Nucleic Acids Res. 2018.
PMID: 29216382
Free PMC article.
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Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells.
Hendel A, Bak RO, Clark JT, Kennedy AB, Ryan DE, Roy S, Steinfeld I, Lunstad BD, Kaiser RJ, Wilkens AB, Bacchetta R, Tsalenko A, Dellinger D, Bruhn L, Porteus MH.
Hendel A, et al. Among authors: lunstad bd.
Nat Biotechnol. 2015 Sep;33(9):985-989. doi: 10.1038/nbt.3290. Epub 2015 Jun 29.
Nat Biotechnol. 2015.
PMID: 26121415
Free PMC article.
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Phosphonoacetate Modifications Enhance the Stability and Editing Yields of Guide RNAs for Cas9 Editors.
Ryan DE, Diamant-Levi T, Steinfeld I, Taussig D, Visal-Shah S, Thakker S, Lunstad BD, Kaiser RJ, McCaffrey R, Ortiz M, Townsend J, Welch WRW, Singh M, Curry B, Dellinger DJ, Bruhn L.
Ryan DE, et al. Among authors: lunstad bd.
Biochemistry. 2023 Dec 19;62(24):3512-3520. doi: 10.1021/acs.biochem.1c00768. Epub 2022 Apr 18.
Biochemistry. 2023.
PMID: 35436085
Free PMC article.
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