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New screening methods for probiotics with adhesion properties to sialic acid and sulphate residues in human colonic mucin using the Biacore assay.
Huang IN, Okawara T, Watanabe M, Kawai Y, Kitazawa H, Ohnuma S, Shibata C, Horii A, Kimura K, Taketomo N, Xiao JZ, Iwatsuki K, Saito T. Huang IN, et al. Among authors: ohnuma s. J Appl Microbiol. 2013 Mar;114(3):854-60. doi: 10.1111/jam.12063. Epub 2013 Jan 7. J Appl Microbiol. 2013. PMID: 23136989 Free article.
Conversion from farnesyl diphosphate synthase to geranylgeranyl diphosphate synthase by random chemical mutagenesis.
Ohnuma S, Nakazawa T, Hemmi H, Hallberg AM, Koyama T, Ogura K, Nishino T. Ohnuma S, et al. J Biol Chem. 1996 Apr 26;271(17):10087-95. doi: 10.1074/jbc.271.17.10087. J Biol Chem. 1996. PMID: 8626566 Free article.
From the libraries, the mutants that showed the activity of geranylgeranyl diphosphate (GGPP) synthase were selected by the red-white screening method (Ohnuma, S.-i., Suzuki, M., and Nishino, T. (1994) J. Biol. Chem. 268, 14792-14797), which utilized carotenoid synt …
From the libraries, the mutants that showed the activity of geranylgeranyl diphosphate (GGPP) synthase were selected by the red-white screen …
229 results