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A transient tobacco expression system coupled to MALDI-TOF-MS allows validation of the impact of differential targeting on structure and activity of a recombinant therapeutic glycoprotein produced in plants.
Mokrzycki-Issartel N, Bouchon B, Farrer S, Berland P, Laparra H, Madelmont JC, Theisen M. Mokrzycki-Issartel N, et al. Among authors: theisen m. FEBS Lett. 2003 Sep 25;552(2-3):170-6. doi: 10.1016/s0014-5793(03)00916-5. FEBS Lett. 2003. PMID: 14527682 Free article.
N-glycosylation potential of maize: the human lactoferrin used as a model.
Samyn-Petit B, Gruber V, Flahaut C, Wajda-Dubos JP, Farrer S, Pons A, Desmaizieres G, Slomianny MC, Theisen M, Delannoy P. Samyn-Petit B, et al. Among authors: theisen m. Glycoconj J. 2001 Jul;18(7):519-27. doi: 10.1023/a:1019640312730. Glycoconj J. 2001. PMID: 12151713
Production of human lactoferrin in transgenic tobacco plants.
Salmon V, Legrand D, Slomianny MC, el Yazidi I, Spik G, Gruber V, Bournat P, Olagnier B, Mison D, Theisen M, Mérot B. Salmon V, et al. Among authors: theisen m. Protein Expr Purif. 1998 Jun;13(1):127-35. doi: 10.1006/prep.1998.0886. Protein Expr Purif. 1998. PMID: 9631525
270 results