Abstract
Four types of phosphorothioate antisense oligonucleotides for transforming growth factor-beta 2 were synthesized and tested for their antisense activity in U937 cells. The full-length phosphorothioate modified antisense analogues exhibited the highest inhibitory effects on the transforming growth factor-beta 2 expression in U937 cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents / chemistry
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Antineoplastic Agents / metabolism
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Antineoplastic Agents / pharmacology
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Blotting, Western
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Cation Exchange Resins / pharmacology
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Cell Division / drug effects
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Codon, Initiator
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Dose-Response Relationship, Drug
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Drug Carriers
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Drug Evaluation, Preclinical
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Fluorescein-5-isothiocyanate / analysis
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Fluorescein-5-isothiocyanate / chemistry
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Humans
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Indicators and Reagents / pharmacology
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Lipids / pharmacology
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Oligonucleotides, Antisense / chemistry*
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Oligonucleotides, Antisense / metabolism
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Oligonucleotides, Antisense / pharmacology*
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Oxidation-Reduction
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Phosphorus Compounds / chemistry
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RNA, Messenger / drug effects
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RNA, Messenger / genetics
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Thionucleotides / chemistry
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Thionucleotides / pharmacology
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Transforming Growth Factor beta / antagonists & inhibitors*
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Transforming Growth Factor beta / genetics
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Transforming Growth Factor beta / metabolism*
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U937 Cells
Substances
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Antineoplastic Agents
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Cation Exchange Resins
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Codon, Initiator
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Drug Carriers
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Indicators and Reagents
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Lipids
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Lipofectamine
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Oligonucleotides, Antisense
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Phosphorus Compounds
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RNA, Messenger
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Thionucleotides
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Transforming Growth Factor beta
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Fluorescein-5-isothiocyanate