Inhibition of transforming growth factor-beta 2 expression with phosphorothioate antisense oligonucleotides in U937 cells

Bioorg Med Chem Lett. 1999 Jan 4;9(1):13-8. doi: 10.1016/s0960-894x(98)00675-1.

Abstract

Four types of phosphorothioate antisense oligonucleotides for transforming growth factor-beta 2 were synthesized and tested for their antisense activity in U937 cells. The full-length phosphorothioate modified antisense analogues exhibited the highest inhibitory effects on the transforming growth factor-beta 2 expression in U937 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / metabolism
  • Antineoplastic Agents / pharmacology
  • Blotting, Western
  • Cation Exchange Resins / pharmacology
  • Cell Division / drug effects
  • Codon, Initiator
  • Dose-Response Relationship, Drug
  • Drug Carriers
  • Drug Evaluation, Preclinical
  • Fluorescein-5-isothiocyanate / analysis
  • Fluorescein-5-isothiocyanate / chemistry
  • Humans
  • Indicators and Reagents / pharmacology
  • Lipids / pharmacology
  • Oligonucleotides, Antisense / chemistry*
  • Oligonucleotides, Antisense / metabolism
  • Oligonucleotides, Antisense / pharmacology*
  • Oxidation-Reduction
  • Phosphorus Compounds / chemistry
  • RNA, Messenger / drug effects
  • RNA, Messenger / genetics
  • Thionucleotides / chemistry
  • Thionucleotides / pharmacology
  • Transforming Growth Factor beta / antagonists & inhibitors*
  • Transforming Growth Factor beta / genetics
  • Transforming Growth Factor beta / metabolism*
  • U937 Cells

Substances

  • Antineoplastic Agents
  • Cation Exchange Resins
  • Codon, Initiator
  • Drug Carriers
  • Indicators and Reagents
  • Lipids
  • Lipofectamine
  • Oligonucleotides, Antisense
  • Phosphorus Compounds
  • RNA, Messenger
  • Thionucleotides
  • Transforming Growth Factor beta
  • Fluorescein-5-isothiocyanate