Immune regulatory loops determine productive interactions within human T lymphocyte-dendritic cell clusters

G Stuhler; A Zobywalski; F Grünebach; P Brossart; V L Reichardt; H Barth; S Stevanovic; W Brugger; L Kanz; S F Schlossman

doi:10.1073/pnas.96.4.1532

Immune regulatory loops determine productive interactions within human T lymphocyte-dendritic cell clusters

Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1532-5. doi: 10.1073/pnas.96.4.1532.

Authors

G Stuhler¹, A Zobywalski, F Grünebach, P Brossart, V L Reichardt, H Barth, S Stevanovic, W Brugger, L Kanz, S F Schlossman

Affiliation

¹ University of Tübingen, Medical Clinic II, Otfried-Müller Strasse 10, 72076 Tübingen, Germany.

Abstract

Help for the induction of cytolytic T lymphocytes is mediated by dendritic cells (DC) that are conditioned by CD40 signaling. We identified tumor necrosis factor family member CD27L/CD70, which is expressed by cytolytic T lymphocytes on interaction with DC to control CD154 (CD40L) up-regulation on CD45RA+ helper T cells for subsequent DC stimulation. The results show that the initiation of a cytolytic immune response is determined by regulatory circuits, requiring simultaneous activation and differentiation of all cells involved in T lymphocyte-DC cluster formation.

MeSH terms

Animals
Antigens, CD / immunology*
B-Lymphocytes / immunology
CD27 Ligand
CD40 Antigens / immunology*
Cells, Cultured
Dendritic Cells / immunology*
HLA-A2 Antigen / immunology
Humans
Immunomagnetic Separation
Leukocyte Common Antigens / immunology
Ligands
Membrane Proteins / immunology*
Mice
Receptors, Immunologic / immunology
Recombinant Proteins / biosynthesis
Signal Transduction
T-Lymphocyte Subsets / immunology*
T-Lymphocytes, Cytotoxic / immunology*
Transfection

Substances

Antigens, CD
CD27 Ligand
CD40 Antigens
CD70 protein, human
Cd70 protein, mouse
HLA-A2 Antigen
Ligands
Membrane Proteins
Receptors, Immunologic
Recombinant Proteins
Leukocyte Common Antigens