Highly sensitive and specific fluorescence reverse transcription-PCR assay for the pseudogene-free detection of beta-actin transcripts as quantitative reference

MeSH terms

• Actins / genetics*
• Base Sequence
• DNA Primers
• Fluorescent Dyes
• Humans
• Pseudogenes*
• RNA, Messenger / genetics*
• Reference Standards
• Reverse Transcriptase Polymerase Chain Reaction / methods*
• Sensitivity and Specificity
• Spectrometry, Fluorescence