A simple method combining the advantages of intraluminal occlusion of the renal artery with hypothermic perfusion of the ischemic organ is presented. Using a double-lumen, balloon-tipped catheter, which is introduced from the periphery, the kidney can be rendered ischemic and can be perfused simultaneously. Initial, hypothermic perfusion and subsequent arrangement on the surface of the open wound effectively protected canine kidneys against ischemic injury over 2 hr of ischemia.