Variation in the sequence of a mitochondrial NADH dehydrogenase I gene fragment among six natural populations of Schistosoma japonicum from China

Int J Parasitol. 1998 Dec;28(12):1931-4. doi: 10.1016/s0020-7519(98)00161-1.

Abstract

The present study investigated the level of genetic variation among Schistosoma japonicum populations of different geographical origins from mainland China. Polymerase chain reaction-based methods were employed to determine the sequence for a subunit of the mitochondrial NADH dehydrogenase I gene for populations from Zhejiang, Anhui, Jiangxi, Hunan, Hubei and Sichuan. No variation was detectable in the NADH dehydrogenase I sequence within populations from Zhejiang and Hubei, whereas sequence variation of 0.2% was detected within populations from Anhui, Jiangxi, Hunan and Sichuan. Pairwise comparison of the sequences representing the six different populations revealed genetic differences ranging from 0 to 0.6%.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • China
  • DNA, Helminth / analysis*
  • DNA, Mitochondrial / analysis*
  • Genetic Variation*
  • Molecular Sequence Data
  • NADH Dehydrogenase / genetics*
  • Polymerase Chain Reaction / methods
  • Polymorphism, Restriction Fragment Length
  • Rabbits
  • Schistosoma japonicum / genetics*
  • Schistosoma japonicum / growth & development
  • Schistosoma japonicum / isolation & purification
  • Schistosomiasis japonica / parasitology*
  • Sequence Analysis, DNA
  • Snails / parasitology

Substances

  • DNA, Helminth
  • DNA, Mitochondrial
  • NADH Dehydrogenase

Associated data

  • GENBANK/AF056291
  • GENBANK/AF056292
  • GENBANK/AF056293
  • GENBANK/AF056294
  • GENBANK/AF056295
  • GENBANK/AF056296