Chitin synthase genes in the arbuscular mycorrhizal fungus Glomus versiforme: full sequence of a gene encoding a class IV chitin synthase

FEMS Microbiol Lett. 1999 Jan 1;170(1):59-67. doi: 10.1111/j.1574-6968.1999.tb13355.x.

Abstract

Chitin synthase genes of the arbuscular mycorrhizal fungus Glomus versiforme were sought in an investigation of the molecular basis of fungal growth. Three DNA fragments (Gvchs1, Gvchs2 and Gvchs3) corresponding to the conserved regions of distinct chitin synthase (chs) genes were amplified by means of the polymerase chain reaction (PCR) with two sets of degenerate primers. Gvchs1 and Gvchs2 encode two class I chitin synthases, whereas Gvchs3 encodes a class IV chitin synthase. A genomic library was used to obtain the Gvchs3 complete gene (1194 amino acids), which shows a very close similarity to the class IV chitin synthase from Neurospora crassa.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chitin Synthase / chemistry
  • Chitin Synthase / genetics*
  • Chitin Synthase / metabolism*
  • Cloning, Molecular
  • DNA Primers
  • DNA, Fungal / analysis
  • Fungi / enzymology
  • Fungi / genetics*
  • Genomic Library
  • Molecular Sequence Data
  • Phylogeny
  • Polymerase Chain Reaction / methods
  • Sequence Analysis, DNA

Substances

  • DNA Primers
  • DNA, Fungal
  • Chitin Synthase

Associated data

  • GENBANK/AJ009628
  • GENBANK/AJ009629
  • GENBANK/AJ009630