CXC chemokines interleukin-8 (IL-8) and growth-related gene product alpha (GROalpha) modulate Purkinje neuron activity in mouse cerebellum

J Neuroimmunol. 1998 Dec 1;92(1-2):122-32. doi: 10.1016/s0165-5728(98)00192-1.

Abstract

We give here evidence that Purkinje neurons (PNs) of mouse cerebellar slices studied with patch clamp technique combined with laser confocal microscopy, respond to human IL-8 and GROalpha by (i) a cytosolic Ca2+ transient compatible with inositol (1,4,5) trisphosphate (InsP3) formation; (ii) an enhancement of the neurotransmitter release; and (iii) an impairment of the long-term depression of synaptic strength (LTD). It was also found the expression of IL-8 receptor type 2 in PN and granule cells by immunofluorescence, immunoblotting and RT-PCR analysis. Considered together these findings suggest that IL-8 and GROalpha may play a neuromodulatory role on mouse cerebellum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Calcium / metabolism
  • Chemokine CXCL1
  • Chemokines, CXC*
  • Chemotactic Factors / pharmacology*
  • Cytosol / metabolism
  • Electric Conductivity
  • Excitatory Postsynaptic Potentials / drug effects
  • Excitatory Postsynaptic Potentials / physiology
  • Fluorescent Dyes
  • Growth Inhibitors / pharmacology*
  • Growth Substances / pharmacology*
  • Humans
  • In Vitro Techniques
  • Inositol 1,4,5-Trisphosphate / metabolism
  • Intercellular Signaling Peptides and Proteins*
  • Interleukin-8 / pharmacology*
  • Long-Term Potentiation / drug effects
  • Mice
  • Mice, Inbred BALB C
  • Organic Chemicals
  • Purkinje Cells / drug effects*
  • Purkinje Cells / metabolism
  • Purkinje Cells / physiology
  • Rats
  • Rats, Wistar
  • Receptors, Chemokine / metabolism
  • Receptors, Interleukin / metabolism
  • Receptors, Interleukin-8B

Substances

  • CXCL1 protein, human
  • Chemokine CXCL1
  • Chemokines, CXC
  • Chemotactic Factors
  • Cxcl1 protein, mouse
  • Cxcl1 protein, rat
  • Fluorescent Dyes
  • Growth Inhibitors
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Interleukin-8
  • Organic Chemicals
  • Receptors, Chemokine
  • Receptors, Interleukin
  • Receptors, Interleukin-8B
  • calcium green
  • Inositol 1,4,5-Trisphosphate
  • Calcium