Purification and characterization of P33 protein of Candida utilis homologous to bgl2p of Saccharomyces cerevisiae; comparative analysis of the role of these proteins in molecular organization of the yeast cell walls

T S Kalebina; D K Laurinavichyute; A B Shevelev; G V Fominov; E I Levitin; O V Alekseeva; S Chzhan; N V Pan'kova; V N Ksenzenko; V M Stepanov; I S Kulaev

Purification and characterization of P33 protein of Candida utilis homologous to bgl2p of Saccharomyces cerevisiae; comparative analysis of the role of these proteins in molecular organization of the yeast cell walls

Biochemistry (Mosc). 1998 Dec;63(12):1419-23.

Authors

T S Kalebina¹, D K Laurinavichyute, A B Shevelev, G V Fominov, E I Levitin, O V Alekseeva, S Chzhan, N V Pan'kova, V N Ksenzenko, V M Stepanov, I S Kulaev

Affiliation

¹ School of Biology, Lomonosov Moscow State University, Moscow, 119899, Russia.

PMID: 9916160

Abstract

P33 protein was isolated from the cell walls of Candida utilis. Homology between P33 and Bgl2p proteins from the cell walls of Saccharomyces cerevisiae was shown. The important role of these proteins in molecular organization of yeast cell walls was demonstrated using trypsin proteolysis and the "gene disruption" method.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Candida / genetics*
Candida / metabolism
Cell Wall / metabolism*
Cloning, Molecular
Fungal Proteins / genetics*
Glucan Endo-1,3-beta-D-Glucosidase / genetics*
Humans
Infant, Newborn
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae / metabolism
Saccharomyces cerevisiae Proteins*
Sequence Homology

Substances

Fungal Proteins
Saccharomyces cerevisiae Proteins
BGL2 protein, S cerevisiae
Glucan Endo-1,3-beta-D-Glucosidase