Objectives were to characterize changes in concentrations of LH, FSH, and estradiol-17beta (estradiol) in blood and populations of ovarian follicles of prepubertal cattle during waves of follicular development and to evaluate interactions between day after follicular aspiration and month prepuberty for these variables. During each month (month prepuberty), ovarian follicles of prepubertal cattle were aspirated to induce synchronous emergence of a wave of follicular development (day after follicular aspiration). Characteristics of ovarian follicular development and concentrations of hormones in blood were evaluated during the synchronous wave of follicular growth. Multiple regression was used to estimate hormonal variables and evaluate interactions for variables between day after follicular aspiration and month prepuberty. There were no interactions between day after follicular aspiration and month prepuberty for numbers of follicles </= 4 or > 5 mm or concentrations of LH, FSH, and estradiol. The pattern of change in these variables after follicular aspiration was, therefore, similar each month prepuberty. There were interactions between day after follicular aspiration and month prepuberty for frequency and amplitude of LH pulses and size of largest follicle. There were also endocrine changes that were related to follicular development after follicular aspiration throughout the peripubertal period.