TOFE human-B-cell-line-based adsorption-inhibition assay to detect HCV neutralizing antibodies

M B Valli; L Bertolini; A Manzin; M Clementi; G Carloni

doi:10.1016/s0923-2516(99)89015-3

TOFE human-B-cell-line-based adsorption-inhibition assay to detect HCV neutralizing antibodies

Res Virol. 1998 Sep-Oct;149(5):331-3. doi: 10.1016/s0923-2516(99)89015-3.

Authors

M B Valli¹, L Bertolini, A Manzin, M Clementi, G Carloni

Affiliation

¹ Istituto di Medicina Sperimentale, Rome, Italy.

PMID: 9879614
DOI: 10.1016/s0923-2516(99)89015-3

Abstract

We previously demonstrated that the human lymphoblastoid B-cell line (LCL) TOFE, derived from normal human bone marrow, is permissive to HCV infection. In this report we developed an in vitro HCV adsorption-inhibition assay based on TOFE cells, to reveal the presence of neutralizing antibodies in sera from acutely infected patients.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adsorption
B-Lymphocytes / virology*
Cell Line
Hepacivirus / isolation & purification
Hepacivirus / physiology*
Hepatitis C / immunology*
Hepatitis C Antibodies / blood*
Hepatitis C Antibodies / immunology
Humans
Neutralization Tests
Reverse Transcriptase Polymerase Chain Reaction

Substances

Hepatitis C Antibodies