Detection of bovine milk proteins in soymilk by Western blotting

J Food Prot. 1998 Dec;61(12):1691-4. doi: 10.4315/0362-028x-61.12.1691.

Abstract

A Western blotting method for the detection of whey milk proteins in commercial soymilks was applied to assess the food safety. Soy proteins and milk proteins were separated by SDS-PAGE in PhastSystem equipment. After the electrophoretic separation, immunodetection with anti-bovine alpha-lactalbumin and anti-bovine beta-lactoglobulin antisera was performed. Adulteration with bovine protein in percentages of 0.1% in soy protein can be detected. Western blotting of bovine alpha-lactalbumin and bovine beta-lactoglobulin was applied to detect adulteration by bovine milk proteins in different soymilks: powdered soymilk and soy infant formulas.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Cattle
  • Electrophoresis, Polyacrylamide Gel
  • Food Contamination* / prevention & control
  • Food Inspection / methods*
  • Food Inspection / standards
  • Glycine max* / metabolism*
  • Infant Food / analysis*
  • Milk Proteins / analysis*
  • Soybean Proteins / analysis

Substances

  • Milk Proteins
  • Soybean Proteins