Structure of sulfated O-specific polysaccharide of the marine bacterium Pseudoalteromonas marinoglutinosa KMM 232

N A Komandrova; S V Tomshich; V V Isakov; L A Romanenko

Structure of sulfated O-specific polysaccharide of the marine bacterium Pseudoalteromonas marinoglutinosa KMM 232

Biochemistry (Mosc). 1998 Oct;63(10):1200-4.

Authors

N A Komandrova¹, S V Tomshich, V V Isakov, L A Romanenko

Affiliation

¹ Pacific Institute of Bioorganic Chemistry, Far East Division of the Russian Academy of Sciences, Vladivostok, 690022, Russia. elnaz@piboc.marine.su

PMID: 9864455

Abstract

A sulfated O-specific polysaccharide containing D-mannose, L-rhamnose, and the sulfate group was obtained by mild acid hydrolysis of lipopolysaccharide (S-form) of the marine bacterium Pseudoalteromonas marinoglutinosa KMM 232. Based on analysis of methylation and 13C-NMR spectroscopy of native and desulfated polysaccharides, the following structure of disaccharide repeat unit in the O-specific polysaccharide has been established: [scheme]. This is the first report of a sulfated O-specific polysaccharide isolated from gram-negative bacteria.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Sequence
Gram-Negative Aerobic Bacteria / chemistry*
Gram-Negative Aerobic Bacteria / immunology
Magnetic Resonance Spectroscopy
Molecular Structure
O Antigens / chemistry*
Polysaccharides, Bacterial / chemistry*
Seawater / microbiology
Sulfates / chemistry

Substances

O Antigens
Polysaccharides, Bacterial
Sulfates