Abstract
To define effects of novel feeding regulating peptides, orexins, in immunocompetent cells, ion channel activity in mouse peritoneal macrophages was analyzed by the perforated patch-clamp method. Orexin-B (OX-B) induced an outward current at smaller holding potentials than K+ equilibrium potentials. Reversal potentials of OX-B induced current were dependent on external K+ concentrations but not on external Cl- concentration. Orexin-A is less effective than OX-B. Quinine blocked the outward current and tetraethylammonium partially suppressed the current. These results suggest that OX-B can modulate macrophage functions through the activation of Ca2+-dependent K2+ channels.
MeSH terms
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Animals
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Calcium / metabolism
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Carrier Proteins / pharmacology
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Charybdotoxin / pharmacology
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Chlorides / metabolism
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Dose-Response Relationship, Drug
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Egtazic Acid / pharmacology
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Female
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Intracellular Signaling Peptides and Proteins*
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Leptin
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Macrophages, Peritoneal / drug effects*
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Macrophages, Peritoneal / physiology
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Male
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Membrane Potentials / drug effects
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Mice
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Neuropeptides / pharmacology*
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Orexins
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Patch-Clamp Techniques
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Potassium / metabolism
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Potassium Channel Blockers
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Potassium Channels / physiology*
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Proteins / pharmacology
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Quinine / pharmacology
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Secretin / pharmacology
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Tetraethylammonium / pharmacology
Substances
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Carrier Proteins
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Chlorides
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Intracellular Signaling Peptides and Proteins
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Leptin
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Neuropeptides
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Orexins
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Potassium Channel Blockers
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Potassium Channels
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Proteins
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Charybdotoxin
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Secretin
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Egtazic Acid
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Tetraethylammonium
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Quinine
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Potassium
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Calcium