Abstract
The effect of potential mediators of mucus secretion was investigated in the isolated vascularly perfused rat colon by using a sandwich enzyme-linked immunosorbent assay for rat colonic mucin and by histochemical analysis. Bethanechol (100-200 microM), bombesin (100 nM), and vasoactive intestinal peptide (VIP, 100 nM) provoked a dramatic mucin discharge (maximal response at 900, 900, and 600% of control loops, respectively). VIP-stimulated mucin secretion was abolished by tetrodotoxin, whereas atropine was without effect. In contrast, both tetrodotoxin and atropine significantly decreased mucin release induced by bombesin. Isoproterenol or calcitonin gene-related peptide was without effect. Serotonin (1-5 microM) and peptide YY (10 nM) evoked mucin discharge, whereas glucagon-like peptide-1 did not release mucin. Finally, bromolasalocid (20 microM), interleukin-1beta (0.25 nM), sodium nitroprusside (1 mM), and dimethyl-PGE2 (2.5 microM) induced mucus discharge. The results demonstrated a good correlation between the immunological method and histological analysis. In conclusion, these findings suggest a role for the enteric nervous system, the enteroendocrine cells, and resident immune cells in mediation of colonic mucus release.
MeSH terms
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16,16-Dimethylprostaglandin E2 / pharmacology
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Animals
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Atropine / pharmacology
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Bethanechol / pharmacology
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Bombesin / pharmacology
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Calcitonin Gene-Related Peptide / pharmacology
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Colon / physiology*
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Enteric Nervous System / drug effects
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Enteric Nervous System / physiology
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Gastrointestinal Hormones / pharmacology*
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Glucagon / pharmacology
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Glucagon-Like Peptide 1
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Inflammation
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Interleukin-1 / pharmacology
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Intestinal Mucosa / cytology
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Intestinal Mucosa / drug effects
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Intestinal Mucosa / physiology*
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Isoproterenol / pharmacology
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Lasalocid / analogs & derivatives
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Lasalocid / pharmacology
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Male
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Mucus / drug effects
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Mucus / metabolism*
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Neurotransmitter Agents / pharmacology*
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Nitroprusside / pharmacology
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Peptide Fragments / pharmacology
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Peptide YY / pharmacology
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Protein Precursors / pharmacology
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Rats
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Rats, Wistar
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Serotonin / pharmacology
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Tetrodotoxin / pharmacology
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Vasoactive Intestinal Peptide / pharmacology
Substances
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Gastrointestinal Hormones
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Interleukin-1
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Neurotransmitter Agents
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Peptide Fragments
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Protein Precursors
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Bethanechol
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Peptide YY
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Nitroprusside
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Serotonin
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Vasoactive Intestinal Peptide
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Tetrodotoxin
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Atropine
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Glucagon-Like Peptide 1
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Glucagon
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Calcitonin Gene-Related Peptide
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Isoproterenol
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16,16-Dimethylprostaglandin E2
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Bombesin
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bromolasalocid
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Lasalocid